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LTBI treatment can prevent and reduce active tuberculosis spread. We examined associations between mental health, social support, and perceptions of general health in 50 LTBI-positive, homeless adults enrolled in LTBI treatment. Depression and anxiety prevalence were 40% and 48%, respectively. Depression was negatively associated with general health, positive social interaction, and tangible, emotional\/informational, and total social support, and positively associated with severe substance use ( ps\u2009&lt;\u20090.05). Anxiety was negatively associated with emotional\/informational, tangible and total social support, and positively associated with severe substance use ( ps\u2009&lt;\u20090.05). 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