{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:22:24Z","timestamp":1777497744551,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Clinical Hemorheology and Microcirculation"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The ischemia-reperfusion injury (IRI) is unavoidable in vascular surgery. Damage to the microcirculation and endothelial glycocalyx might set up a shock with loss of circulatory coherence and organ failure. Sulodexide may help to protect endothelial glycocalyx and alleviate the ischemia-reperfusion injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty female piglets underwent surgery with a 30-min-long suprarenal aortic clamp, followed by two hours of reperfusion. Ten piglets received sulodexide before the clamp, and 10 received normal saline. Blood and urine samples were taken at baseline and in 20-min intervals until the 120\n                      <jats:sup>th<\/jats:sup>\n                      minute to analyze the serum syndecan-1, E-selectin, and thrombomodulin. Albumin and glycosaminoglycans were examined in the urine. The kidney biopsies before and after the protocol were examined by light microscopy with hematoxylin-eosin staining. The sublingual microcirculation was recorded by side-stream dark field imaging at the time as blood and urine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Based on the 2-way ANOVA testing, there was no statistically significant difference in the parameters of sublingual microcirculation. Serum markers of endothelial cell activation and damage (E-selectin and thrombomodulin) did not show any statistically significant difference either. Syndecan-1, a marker of glycocalyx damage, showed statistically significantly higher values based on the 2-way ANOVA testing (p\u2009&lt;\u20090.0001) with the highest difference in the 80\n                      <jats:sup>th<\/jats:sup>\n                      minute: 7.8 (3.9\u201344) ng\/mL in the control group and 1.8 (0.67\u20132.8) ng\/mL in the sulodexide group. In the urine, the albuminuria was higher in the control group, although not statistically significant. Glycosaminoglycans were statistically significantly higher in the sulodexide group based on the mixed-effect analysis due to the intervention itself. Histological analysis of the renal biopsies showed necrosis in both groups after reperfusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Administering sulodexide significantly reduced the level of endothelial markers of IRI. The study results support further research into using preemptive administration of sulodexide to modulate IRI in clinical medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13860291241306568","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T01:21:36Z","timestamp":1739496096000},"page":"260-269","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevention of ischemia-reperfusion injury on the porcine model of supra-renal aortic clamp by sulodexide\n                    <sup\/>"],"prefix":"10.1177","volume":"89","author":[{"given":"Ludek","family":"Hana","sequence":"first","affiliation":[{"name":"Department of Military Surgery, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"},{"name":"Department of Surgery, 2nd Faculty of Medicine, Charles University and Military University Hospital Prague, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Tlapakova","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Cizkova","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Ticha","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Cerny","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada"},{"name":"Department of Anesthesiology, Perioperative Medicine, and Intensive Care Medicine, University of J. 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Purkyne in Usti nad Labem, Masaryk Hospital in Usti nad Labem, Usti nad Labem, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"},{"name":"Department of Anaesthesia and Intensive Care Medicine, Charles University, 3rd Faculty of Medicine, Prague, Czech Republic"},{"name":"Faculty of Health Sciences, Technical University in Liberec, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert G","family":"Hahn","sequence":"additional","affiliation":[{"name":"Karolinska Institutet at Danderyds Hospital (KIDS), Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaromir","family":"Koci","sequence":"additional","affiliation":[{"name":"Department of Military Surgery, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"},{"name":"Department of Emergency Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Astapenko","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Resuscitation, and Intensive Care Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic"},{"name":"Faculty of Medicine in Hradec Kralove, Charles University, Prague, Czech Republic"},{"name":"Faculty of Health Sciences, Technical University in Liberec, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3233\/CH-242142"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3233\/CH-1663301"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc14726"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3233\/CH-242128"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252010891"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2017.1379943"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.684852"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-024-02776-4"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00540-016-2195-0"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2017.10.002"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11020177"},{"key":"e_1_3_4_13_2","first-page":"198","article-title":"Protective role of nitric oxide donors on endothelium in ischemia-reperfusion injury: a meta-analysis of randomized controlled trials","volume":"23","author":"Dou C","year":"2023","unstructured":"Dou C, Han X, Xie H, et al. 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