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We investigated the effect of short-term storage on the stability of RBC aggregation and deformability. Blood was obtained from apparently healthy volunteers and anticoagulated with lithium heparin or ethylenediaminetetraacetic acid (EDTA). Samples were stored either at ambient (22\u2005\u00b0C) or cold (4\u2005\u00b0C) temperature for up to 48\u2005h. Aggregation and deformability were measured utilising light transmission and laser backscatter methods, and laser diffractometry, respectively. EDTA significantly lowered aggregation when compared with heparin-anticoagulated blood. During ambient temperature storage, EDTA preserved baseline RBC aggregation for up to 4\u2005h, while lithium heparin-anticoagulated blood showed significant changes within 1\u2005h. Deformability was altered after 8\u2005h of storage, irrespective of anticoagulant. Cold storage extended stability most effectively in EDTA. It remains that EDTA should be the anticoagulant of choice to preserve blood physical properties. Even short-term storage of blood has a remarkable impact on physical properties; when unavoidable, however, EDTA blood should be stored at 4\u2005\u00b0C for same day testing without negative effect.<\/jats:p>","DOI":"10.1177\/13860291251376503","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T17:53:47Z","timestamp":1758045227000},"page":"3-13","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Storage temperature and anticoagulant choice determine the short-term stability of blood rheological parameters"],"prefix":"10.1177","volume":"91","author":[{"given":"Lennart","family":"Kuck","sequence":"first","affiliation":[{"name":"Biorheology Research Laboratory, Griffith University, Gold Coast, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco A","family":"Frappa","sequence":"additional","affiliation":[{"name":"Alcor Scientific, Smithfield, RI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony P","family":"McNamee","sequence":"additional","affiliation":[{"name":"Biorheology Research Laboratory, Griffith University, Gold Coast, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Biorheology Research Laboratory, Griffith University, Gold Coast, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2405086121"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3233\/CH-2009-1178"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00288"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.49.030187.001141"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1989.tb00296.x"},{"key":"e_1_3_3_7_2","first-page":"27","article-title":"Red blood cell storage lesion: causes and potential clinical consequences","volume":"17","author":"Yoshida T","year":"2019","unstructured":"Yoshida T, Prudent M, D'Alessandro A. 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