{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:28:46Z","timestamp":1777397326801,"version":"3.51.4"},"reference-count":60,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Immunotec, Inc. Quebec, CA"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Deficits in Reelin expression play a significant role in the pathogenesis of various neurological disorders, including schizophrenia and Alzheimer's disease (AD). Notably, Reelin-expressing neurons of the entorhinal cortex layer II are among the first to be affected in AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Strategies aimed at correcting deficits in Reelin might provide a novel therapeutic approach for AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we examined the effects of the whey protein supplement and glutathione (GSH) precursor, Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      , on Reelin expression both in vitro in hippocampal-entorhinal cortex slices from rat brain and in vivo in the hA\u03b2PP\n                      <jats:sub>SweInd<\/jats:sub>\n                      (J20) mouse model of AD\n                      <jats:italic>.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Incubation of brain slices with Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      increased Reelin expression at the mRNA and protein levels. Oral treatment with Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      , given\n                      <jats:italic>ad libitum<\/jats:italic>\n                      in drinking water beginning at 3 months of age, corrected a deficit in cortical GSH levels observed in untreated mice and preserved Reelin expression in the hippocampal-entorhinal cortex sub-region of 5-month-old J20 mice. We also assessed the long-term effects of Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      by treating J20 mice from 3 months old to 12 months old. Long-term Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      treatment preserved brain GSH and rescued Reelin mRNA and protein expression, while significantly reducing amyloid plaque formation in the entorhinal cortex and hippocampus of AD mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings suggest that Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      promotes Reelin expression in vitro and sustains brain GSH and Reelin expression while diminishing amyloid plaque load in the entorhinal cortex and hippocampus of J20 mice. Thus, Immunocal\n                      <jats:sup>\u00ae<\/jats:sup>\n                      offers a promising therapeutic approach to enhance Reelin expression and curtail amyloid deposition in AD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13872877251338323","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T02:59:17Z","timestamp":1746500357000},"page":"94-110","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunocal\n                    <sup>\u00ae<\/sup>\n                    , a cysteine-rich whey protein, rescues reelin and reduces amyloid plaque burden in a transgenic amyloid-\u03b2 protein precursor (hA\u03b2PP\n                    <sub>SweInd<\/sub>\n                    ) mouse model of Alzheimer's disease"],"prefix":"10.1177","volume":"106","author":[{"given":"Alexandra","family":"Sandberg","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srivalli","family":"Puttagunta","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Duval","sequence":"additional","affiliation":[{"name":"Knoebel Institute for Healthy Aging, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holly","family":"Fleming","sequence":"additional","affiliation":[{"name":"Knoebel Institute for Healthy Aging, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilia","family":"Koza","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kade","family":"Hieber","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5968-4695","authenticated-orcid":false,"given":"Jessica","family":"Holsopple","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Reyna","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, 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