{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T07:48:33Z","timestamp":1785743313976,"version":"3.56.0"},"reference-count":51,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Alzheimer's disease (AD) is a neurodegenerative disease. In previous study, we discovered that methanol extract of miracle fruits seeds (MFSs) had a therapeutic effect on AD model mice.\n                    <jats:bold>Objective:<\/jats:bold>\n                    In this research, we aimed to study the role of MFSs in alleviating amyloid-\u03b2 (A\u03b2) deposition and further explore the possible mechanism of action of MFSs.\n                    <jats:bold>Methods:<\/jats:bold>\n                    We used behavioral tests and various immunolabeling techniques on two AD model (A\u03b2 transgenic model strain CL4176 of Caenorhabditis elegans and the 3\u2009\u00d7\u2009Tg-AD mice).\n                    <jats:bold>Results:<\/jats:bold>\n                    In CL4176, MFSs (10\u2005\u03bcg\/mL) could effectively alleviate the paralysis caused by A\u03b2, the A\u03b2 plaques were significantly reduced, the transcription levels of\n                    <jats:italic toggle=\"yes\">A\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">age-1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">akt-1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">mTOR<\/jats:italic>\n                    genes were significantly downregulated, and the transcription of autophagy-related gene\n                    <jats:italic toggle=\"yes\">lgg-1<\/jats:italic>\n                    was enhanced. The cognitive function of mice in the MFSs group was improved, which was related to reduced synapse loss and A\u03b2-positive neurons in the brain. Meanwhile, the transcription levels of\n                    <jats:italic toggle=\"yes\">amyloid-\u03b2 protein precursor (A\u03b2PP)<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Tau<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Akt<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">mTOR<\/jats:italic>\n                    were significantly reduced. The protein expressions of PSD95, SYN1, LC3II\/I and Beclin-1 were significantly increased, and the value of p62\/SQSTM1, p-AKT\/AKT, p-mTOR\/mTOR were reduced.\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    MFSs significantly alleviates A\u03b2 deposition in CL4176 and mice. The mechanism by which it improves cognitive function in AD mice may be related to enhanced autophagy regulated by the Akt\/mTOR signaling pathway.\n                  <\/jats:p>","DOI":"10.1177\/13872877251352545","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T05:15:39Z","timestamp":1751260539000},"page":"1615-1628","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Methanol extract from miracle fruit seeds mitigates Alzheimer's disease and enhance autophagy via suppressing the AKT\/mTOR signal pathway"],"prefix":"10.1177","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6755-2251","authenticated-orcid":false,"given":"Zi-ting","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2388-238X","authenticated-orcid":false,"given":"Xue-yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1623-5969","authenticated-orcid":false,"given":"Liu-lin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6849-7928","authenticated-orcid":false,"given":"Zu-cai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3734-9537","authenticated-orcid":false,"given":"He-xu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4529-9750","authenticated-orcid":false,"given":"Chang-yin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2017.12.006"},{"key":"e_1_3_2_3_2","article-title":"Estimation of the global prevalence of dementia in 2019 and forecasted prevalence in 2050: an analysis for the global burden of disease study 2019","volume":"7","author":"GBD 2019 Dementia Forecasting Collaborators","year":"2022","unstructured":"GBD 2019 Dementia Forecasting Collaborators. 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