{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T17:49:12Z","timestamp":1789235352412,"version":"build-2803163510"},"reference-count":39,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T00:00:00Z","timestamp":1752969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD) is characterized by complex pathological mechanisms, with pyroptosis potentially contributing to neuroinflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify pyroptosis-related genes (PRGs) in AD and explore their role in neuroinflammation, aiming to provide potential biomarkers and therapeutic targets for precision medicine in AD treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transcriptomic data from AD brain tissues (GEO database) were analyzed using multi-omics integration and machine learning. Key PRGs were screened via weighted gene co-expression network analysis (WGCNA), LASSO regression, random forest, and SVM-RFE algorithms. Molecular subtypes and therapeutic potential were assessed through unsupervised clustering and molecular docking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Analysis identified 609 differentially expressed genes (DEGs), with upregulated genes enriched in DNA transcription and mitosis-related pathways. Six core PRGs (MIB1, TUG1, GATA1, CA1, CFH, IL17A) demonstrated strong diagnostic accuracy (AUC\u2009&gt;\u20090.85). Unsupervised clustering revealed two AD subtypes: a high-risk subtype with activated pyroptosis-inflammatory pathways and distinct immune microenvironment features (p\u2009&lt;\u20090.05). Molecular docking confirmed stable binding between CFH and the anti-AD drug candidate fludrocortisone (binding energy\u2009&lt;\u2009\u22127 kcal\/mol).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Pyroptosis modulates neuroinflammation to drive AD progression. CFH and other PRGs serve as promising biomarkers and therapeutic targets, advancing precision strategies for AD subtyping and intervention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13872877251360033","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T05:13:47Z","timestamp":1753074827000},"page":"361-373","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Application of machine learning reveals diagnostic biomarkers related to pyroptosis in Alzheimer's disease and analysis of immune infiltration"],"prefix":"10.1177","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1701-1085","authenticated-orcid":false,"given":"Yujuan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Medical College of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruping","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Fourth Hospital of Changsha\/Changsha Hospital of Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Endocrinology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,20]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.602781"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-021-02449-2"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2011.2"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S200490"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12321"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21218144"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25245789"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2012.11.007"},{"key":"e_1_3_4_10_2","first-page":"1598","article-title":"Alzheimer\u2019s disease facts and figures","volume":"19","author":"Alzheimer's Association","year":"2023","unstructured":"Alzheimer's Association. 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