{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:16:49Z","timestamp":1780669009988,"version":"3.54.1"},"reference-count":38,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Grants-in-Aid for Scientific Research C","award":["23K06811"],"award-info":[{"award-number":["23K06811"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Amyloid-\u03b2 peptides (A\u03b2) applied to Alzheimer's disease (AD) brain bind to amyloid plaques (APs) and this binding is saturable. Meanwhile, anti-immunoglobulin G (IgG) antibodies label APs. Considering the saturable binding of A\u03b2 to APs and the localization of IgGs in APs, specific binding proteins (BPs) for the applied A\u03b2 could be anti-A\u03b2 IgG antibodies (anti-A\u03b2Abs) in APs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Our objective is to demonstrate whether anti-A\u03b2Abs are the BPs for administered A\u03b2 in APs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To identify the binding specificity, we established a tissue competition assay utilizing the competitiveness of ligand specificity to BPs. Biotinylated A\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      (Bio42) was applied as ligand to AD brain sections. Second, Bio42 and IgG (anti-A\u03b2Abs) in APs were observed by double immunofluorescent labeling with ultrahigh-resolution imaging. Finally, to demonstrate the direct binding of Bio42 to anti-A\u03b2Abs we employed immunoprecipitation-western blotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      With the tissue competition assay, binding of Bio42 to APs was competitively blocked by coincubation with competitive A\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      (Pep42) in a dose-dependent manner but not with non-competitive A\u03b2\n                      <jats:sub>40<\/jats:sub>\n                      (Pep40). Colocalization of Bio42 and anti-A\u03b2Abs was clearly detected with ultrahigh-resolution imaging. Anti-A\u03b2Abs were immunoprecipitated by Bio42 and immunoblotted by anti-IgG antibodies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We have provided evidence that the applied A\u03b2 specifically binds to anti-A\u03b2Abs rather than aggregating with A\u03b2 in APs. Our results support the scenario that anti-A\u03b2Abs surround and\/or sequester A\u03b2 from biological fluids to APs, thereby affecting the amount of A\u03b2 in body fluids. It might be important to consider anti-A\u03b2Abs in APs for more effective diagnosis and therapy for AD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13872877251389631","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:16:36Z","timestamp":1761844596000},"page":"1614-1625","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["One of the binding proteins for administered amyloid-\u03b2 appears to be anti-A\u03b2 IgG antibody in amyloid plaques"],"prefix":"10.1177","volume":"108","author":[{"given":"Reisuke H","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Anatomic Pathology, Tokyo Medical University, Shinjuku, Tokyo, Japan"},{"name":"Laboratory of Structural Neuropathology, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, 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