{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:14:31Z","timestamp":1783415671314,"version":"3.54.6"},"reference-count":9,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2025ZD0214700"],"award-info":[{"award-number":["2025ZD0214700"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101401"],"award-info":[{"award-number":["2021YFA1101401"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["25ZR1402459"],"award-info":[{"award-number":["25ZR1402459"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500850"],"award-info":[{"award-number":["32500850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32571147"],"award-info":[{"award-number":["32571147"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20292"],"award-info":[{"award-number":["U22A20292"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD) involves white matter deterioration, but how amyloid-\u03b2 (A\u03b2) affects oligodendrocyte lineage cells at different maturation stages remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine whether A\u03b2 impairs oligodendrocyte differentiation and myelination in adult oligodendrocyte precursor cells (OPCs) and to identify molecular correlates via RNA-seq.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Oligodendrocyte lineage cells were examined in plaque-associated regions of 8-month-old 5x familial Alzheimer's disease (FAD) mice by immunohistochemistry. Primary OPCs from neonatal and adult rats were cultured with or without amyloid-\u03b2\n                      <jats:sub>1\u201342<\/jats:sub>\n                      oligomers (oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      ) to assess differentiation. Myelination was evaluated in organotypic slice cultures. RNA-seq and qPCR were performed to identify oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      -induced gene expression changes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In 5xFAD mice, Olig2\n                      <jats:sup>+<\/jats:sup>\n                      cells were reduced near plaques, with CC1\n                      <jats:sup>+<\/jats:sup>\n                      mature oligodendrocytes showing a pronounced decrease, while PDGFR\u03b1\n                      <jats:sup>+<\/jats:sup>\n                      OPCs remained unchanged. In vitro, oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      inhibited differentiation of both neonatal and adult OPCs, with adult OPCs exhibiting intrinsically slower maturation. Slice cultures revealed selective hypomyelination (reduced myelin basic protein) after oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      treatment. RNA-seq showed that oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      induced a distinct transcriptomic profile in adult OPCs, with upregulated genes enriched in immune\/inflammatory pathways. Core inflammatory genes Nr4a1 and Tnf were significantly upregulated, validated by qPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      plaque pathology is associated with oligodendrocyte maturation blockade. A\u03b2 impairs OPC differentiation in purified cultures accompanied by inflammatory transcriptional changes. These findings highlight oligodendrocyte dysfunction in AD white matter pathology and reveal a specific oA\u03b2\n                      <jats:sub>42<\/jats:sub>\n                      response in adult OPCs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13872877261451403","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T08:44:36Z","timestamp":1779698676000},"page":"852-860","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Amyloid-\u03b2 suppresses oligodendrocyte differentiation in adult oligodendrocyte precursor cells (OPCs), with inflammatory gene changes distinguishing it from developmental OPCs"],"prefix":"10.1177","volume":"112","author":[{"given":"Daopeng","family":"Li","sequence":"first","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"},{"name":"State Key Laboratory of Cellular Stress Biology, School of Life Science, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiu","family":"Dai","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangying","family":"Li","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongda","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Medical University"},{"name":"Neurosurgery Department, the First Affiliated Hospital of Ningbo University, Ningbo, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanghanchen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Wang","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4528-2403","authenticated-orcid":false,"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.10.021"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7176"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201709069"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1484-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI155096"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.02.018"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/0115672050401966250625171338"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-025-10236-z"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-026-02620-9"}],"container-title":["Journal of Alzheimer\u2019s 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