{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T14:34:35Z","timestamp":1793025275722,"version":"4.5.0"},"reference-count":34,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca Corrente 2025-2027"],"award-info":[{"award-number":["Ricerca Corrente 2025-2027"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca Finalizzata (GR-2021-12374436)"],"award-info":[{"award-number":["Ricerca Finalizzata (GR-2021-12374436)"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Alzheimer\u2019s Disease"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Plasma phosphorylated tau 217 (pTau217) has emerged as one of the most accurate biomarkers for Alzheimer's disease (AD). However, direct comparisons of pTau217 levels between different automated immunoassays remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>In this context, our work aimed to compare Lumipulse and Ella platforms for the measurement of plasma pTau217 across the AD continuum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were collected from 32 subjective cognitive decline (SCD), 25 mild cognitive impairment (MCI), and 10 Alzheimer's disease (AD) patients. Plasma pTau217 levels were assessed using Lumipulse G600II automated system and Ella immunoassay SR-X platform. Associations with clinical variables and agreement between platforms were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Plasma pTau217 levels increased from SCD to AD with both assays. Lumipulse showed a significant difference between SCD and AD (p\u2009=\u20090.0011), while Ella detected differences between SCD and MCI (p\u2009=\u20090.0198) and SCD and AD (p\u2009=\u20090.0001). Both assays showed a significant inverse correlation with Mini-Mental State Examination (Lumipulse: r\u2009=\u2009\u22120.4913; p\u2009=\u20090.0001. Ella: Pearson r\u2009=\u2009\u22120.5139; p\u2009=\u20090.0001). A moderate correlation between platforms was observed. Bland\u2013Altman analysis revealed a negative bias, with Lumipulse yielding lower values than Ella and increasing variability at higher concentrations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Lumipulse and Ella provided consistent qualitative information on plasma pTau217 across the AD continuum and show similar associations with cognitive performance, meaning that they are both comparable techniques. However, systematic bias and proportional variability limit their quantitative interchangeability, highlighting the need for cross-platform standardization.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/13872877261500491","type":"journal-article","created":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:34:13Z","timestamp":1792816453000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["pTau217 immunoassays in Alzheimer's disease continuum: A comparative study of Ella and Lumipulse"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2689-1592","authenticated-orcid":false,"given":"Evelyne","family":"Minucchi","sequence":"first","affiliation":[{"name":"University of Pavia","place":["Italy"]},{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalinda","family":"Di Gerlando","sequence":"additional","affiliation":[{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Dragoni","sequence":"additional","affiliation":[{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7747-806X","authenticated-orcid":false,"given":"Camillo","family":"Imbimbo","sequence":"additional","affiliation":[{"name":"University of Pavia","place":["Italy"]},{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Zardini","sequence":"additional","affiliation":[{"name":"University of Pavia","place":["Italy"]},{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Cotta Ramusino","sequence":"additional","affiliation":[{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Costa","sequence":"additional","affiliation":[{"name":"University of Pavia","place":["Italy"]},{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella","family":"Gagliardi","sequence":"additional","affiliation":[{"name":"IRCCS Mondino","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,23]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076518"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2020.117676"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2019.04.014"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00361-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40120-024-00658-x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12841"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14073"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.12134"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2023.5319"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14397"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-019-0550-8"},{"key":"e_1_3_3_13_2","article-title":"2025 Alzheimer\u2019s disease facts and figures","volume":"21","author":"Alzheimer\u2019s Association","year":"2025","unstructured":"Alzheimer\u2019s Association. 2025 Alzheimer\u2019s disease facts and figures. 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Mini-Mental State Examination (MMSE) for the detection of dementia in clinically unevaluated people aged 65 and over in community and primary care populations. 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