{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:36:29Z","timestamp":1792899389921,"version":"4.5.0"},"reference-count":24,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Helsinki University Hospital Research Fund","id":[{"id":"https:\/\/ror.org\/02e8hzf44","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Finnish Dental Foundation"},{"name":"Helsinki University Library"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tumour Biol."],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adenoid cystic carcinoma (ACC) of the salivary glands has a poor long-term prognosis. Independent prognostic factors of ACC include histological and clinicopathological features, while a validated gold-standard prognostic immunohistochemical markers are still lacking. Functions of cell adhesion molecule E-cadherin which maintain epithelial integrity, and cell cycle and function regulators nuclear protein Cyclin D1 and transmembrane heparan sulfate proteoglycan syndecan-2 in ACC have not been studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aim of this study was to investigate the immunohistochemical expression of E-cadherin, syndecan-2, and cyclin D1 in ACC of the minor salivary glands. Our objective was to evaluate the prognostic relevance of these markers in relation to disease progression and patient outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study included 46 patients with minor salivary gland ACC treated at the Department of Otorhinolaryngology\u2013Head and Neck Surgery, Helsinki University Hospital, Finland, between 1974 and 2012. Formalin-fixed paraffin-embedded tumor tissue samples were used for immunohistochemical analysis. Staining intensity was scored and associated with clinicopathological parameters and survival data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Immunopositivity varied among tumors, sometimes exhibiting heterogeneous expression within the same tumor. We found moderate to high E-cadherin expression to significantly associate with improved overall (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0,024) and disease-specific survival (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.005). Additionally in multivariate analysis, moderate to high E-cadherin associated with better disease-specific survival with hazard ratio of 0.219 (95% CI 0.065\u20130.734,\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.014). Notably, all four patients with neck recurrences demonstrated low syndecan-2 positivity (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.023). Cyclin D1 did not associate significantly with survival.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, all the studied markers were expressed in the minor salivary gland ACC. E-cadherin showed potentiality as a prognostic immunohistochemical biomarker for survival analysis. Low syndecan-2 expression may be indicative for regional recurrence. Validation in larger multicenter cohorts is warranted to confirm these observations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/14230380261497644","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:51:57Z","timestamp":1792608717000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Prognostic value of E-cadherin, syndecan-2, and cyclin D1 immunohistochemistry in minor salivary gland adenoid cystic carcinoma: A cohort study"],"prefix":"10.1177","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9458-8602","authenticated-orcid":false,"given":"Aleksi","family":"Rytk\u00f6nen","sequence":"first","affiliation":[{"name":"University of Turku and Turku University Hospital"},{"name":"Oulu University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auli","family":"Suominen","sequence":"additional","affiliation":[{"name":"University of Turku"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0451-2404","authenticated-orcid":false,"given":"Antti","family":"M\u00e4kitie","sequence":"additional","affiliation":[{"name":"University of Helsinki"},{"name":"Helsinki University Hospital"},{"name":"Karolinska Institutet and Karolinska University Hospital"},{"name":"Faculty of Medicine, University of Helsinki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0456-4965","authenticated-orcid":false,"given":"Caj","family":"Haglund","sequence":"additional","affiliation":[{"name":"University of Helsinki"},{"name":"University of Helsinki and Helsinki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna K.","family":"Laine","sequence":"additional","affiliation":[{"name":"University of Helsinki"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6079-7881","authenticated-orcid":false,"given":"Jaana","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"University of Turku and Turku University Hospital"},{"name":"University of Helsinki"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/hed.28132"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000918"},{"key":"e_1_3_6_4_2","first-page":"e289405","article-title":"Tumor classification of adenoid cystic carcinoma: systematic review and meta-analysis","volume":"85","author":"On\u00f3rio DCS","year":"2025","unstructured":"On\u00f3rio DCS, Nunes LBV, Nunes BMMB, et al. 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