{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T21:56:26Z","timestamp":1777586186338,"version":"3.51.4"},"reference-count":18,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Eur J Mass Spectrom (Chichester)"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:p>Tiotropium Bromide is a long-acting bronchodilator that is used in the treatment of chronic obstructive pulmonary disease (COPD) and asthma bronchodilator or bronchiolitis, which are substances that expand the bronchi and reduce resistance in the respiratory tract and increase airflow to the lungs. For Tiotropium Bromide found in inhaler capsules to treat COPD, determining the relevant impurities G and H, which are not UV active, is crucial. For this purpose, a new and sensitive liquid chromatography triple-quadrupole mass spectrometry (LC-MS\/MS) detection with electrospray ionization by using multiple reaction monitoring in the positive mode method was developed and validated. The identity of the compounds was supported by using LC-Q\/TOF. All chromatographic studies were performed with a Zorbax Eclipse XDB-C8 (150\u2005mm x 4.6\u2005mm, 5.0\u2005\u00b5m) column with a total injection time of 13\u2005min at a flow rate of 0.4\u2005ml\/min as a gradient. The limit of detection (LOD) and limit of quantitation (LOQ) in the current study range were determined as 1.0\u2005ppb and 2.5\u2005ppb, respectively. The results of the validation parameters following the ICH Q2(R1) guideline were determined within the acceptance criteria.<\/jats:p>","DOI":"10.1177\/14690667231217879","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T01:46:25Z","timestamp":1701395185000},"page":"76-83","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous quantification of tiotropium bromide impurities G\u2009+\u2009H in capsule formulation by LC-MS\/MS"],"prefix":"10.1177","volume":"30","author":[{"given":"Serdar","family":"Erkol","sequence":"first","affiliation":[{"name":"Faculty of Art and Science, Department of Chemistry, Yildiz Technical University, Esenler, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00fcge","family":"G\u00fcleli","sequence":"additional","affiliation":[{"name":"R&D Center, World Medicine \u0130la\u00e7 San. Tic. A.\u015e, Ba\u011fc\u0131lar, \u0130stanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2345-0242","authenticated-orcid":false,"given":"Cem","family":"\u00c7al\u0131\u015fkan","sequence":"additional","affiliation":[{"name":"R&D Center, World Medicine \u0130la\u00e7 San. Tic. A.\u015e, Ba\u011fc\u0131lar, \u0130stanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sevgi","family":"Kocaoba","sequence":"additional","affiliation":[{"name":"Faculty of Art and Science, Department of Chemistry, Yildiz Technical University, Esenler, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"bibr1-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3021"},{"key":"bibr2-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/46.5.445"},{"key":"bibr3-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1177\/0091270003261315"},{"key":"bibr4-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-016-0043-6"},{"key":"bibr5-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2011.10.046"},{"key":"bibr6-14690667231217879","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.1204-06"},{"key":"bibr7-14690667231217879","first-page":"1","volume":"4","author":"Kachave RN","year":"2013","journal-title":"Inventi Rapid: Pharm Analysis & Quality Assurance"},{"key":"bibr8-14690667231217879","doi-asserted-by":"publisher","DOI":"10.31531\/2581-4745.1000107"},{"key":"bibr9-14690667231217879","unstructured":"Tiotropium Bromide\n                      . British Pharmacopoeia, 2022."},{"key":"bibr10-14690667231217879","unstructured":"Tiotropium Bromide\n                      . European Pharmacopoeia 10.7, \n01\/2018:2420."},{"key":"bibr11-14690667231217879","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-017-3413-0"},{"key":"bibr12-14690667231217879","doi-asserted-by":"publisher","DOI":"10.4155\/bio.15.256"},{"key":"bibr13-14690667231217879","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.galenos.2021.77177"},{"key":"bibr14-14690667231217879","first-page":"1327","volume":"46","author":"Zhou YM","year":"2015","journal-title":"Chinese Journal of Pharmaceuticals"},{"key":"bibr15-14690667231217879","unstructured":"ICH Q3B(R2)\n                      . Impurities in New Drug Products, Current Step 4 version, 2 June 2006."},{"key":"bibr16-14690667231217879","unstructured":"ICH Q2(R1)\n                      . Validation of Analytical Procedures: Text and Methodology, Current Step 4 version, 27 October 1994."},{"key":"bibr17-14690667231217879","unstructured":"USP <736>\n                      . 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