{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:17:00Z","timestamp":1788430620532,"version":"build-2803163510"},"reference-count":21,"publisher":"SAGE Publications","issue":"5-6","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Eur J Mass Spectrom (Chichester)"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    The dimer and trimer structures of the non-amyloidogenic rat islet amyloid polypeptide 21\u201337 peptide, formed in an H\n                    <jats:sub>2<\/jats:sub>\n                    O\/CH\n                    <jats:sub>3<\/jats:sub>\n                    OH (1% CH\n                    <jats:sub>3<\/jats:sub>\n                    COOH) solution were investigated using electrospray ionization-tandem mass spectrometry (ESI-MS\/MS). The dissociation of monomers, dimers, and trimers was investigated by MS\/MS using collision-induced dissociation. The peptide bond dissociation between L\n                    <jats:sub>7<\/jats:sub>\n                    and P\n                    <jats:sub>8<\/jats:sub>\n                    was mainly observed in the tandem mass spectra of the monomers and oligomers, regardless of the parent ion charge state. The fragment ions were observed as a series of b\n                    <jats:sub>u<\/jats:sub>\n                    (u\u2009=\u20093\u20134, 6\u20137, 12) or y\n                    <jats:sub>n<\/jats:sub>\n                    (n\u2009=\u200910\u201311, 13\u201314) in the [Mono\u2009+\u20092H]\n                    <jats:sup>2+<\/jats:sup>\n                    (=[monomer\u2009+\u20092H]\n                    <jats:sup>2+<\/jats:sup>\n                    ) tandem mass spectrum. MS\/MS analysis of the [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    (=[dimer\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    ) complex indicated that [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    comprised [Mono\u2009+\u2009H]\n                    <jats:sup>1+<\/jats:sup>\n                    and [Mono\u2009+\u20092H]\n                    <jats:sup>2+<\/jats:sup>\n                    subunits. During covalent bond dissociation of the [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    complex, a fragmentation pattern was observed in the form of {mono\u2009+\u2009(fragment ion of [Mono\u2009+\u20092H]\n                    <jats:sup>2+<\/jats:sup>\n                    )}, resulting from the collision energy dissociation of the [Mono\u2009+\u20092H]\n                    <jats:sup>2+<\/jats:sup>\n                    peptide. The [(C-terminal)\u2013(C-terminal)] interaction geometry was proposed for the [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    complex based on the observation of [y\n                    <jats:sub>10\u2009<\/jats:sub>\n                    +\u2009y\n                    <jats:sub>n<\/jats:sub>\n                    ]\n                    <jats:sup>2+<\/jats:sup>\n                    (n\u2009=\u200910\u201311, 13\u201316) fragment ions in the [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    tandem mass spectrum. MS\/MS analysis of the [Tri\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    (=[trimer\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    ) complex indicated that [Tri\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    comprised [Mono\u2009+\u2009H]\n                    <jats:sup>1+<\/jats:sup>\n                    and [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    subunits. The (monomer\u2013[Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    )\n                    <jats:sup>4+<\/jats:sup>\n                    complex geometry was assumed to be stable based on the presence of {mono\u2009+\u2009(fragment ion of [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    )} ions in the tandem mass spectrum of the [Tri\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    complex. The two [Mono\u2009+\u2009(y\n                    <jats:sub>10\u2009<\/jats:sub>\n                    +\u2009y\n                    <jats:sub>10<\/jats:sub>\n                    )]\n                    <jats:sup>2+<\/jats:sup>\n                    and [Mono\u2009+\u2009(Mono\u2009+\u2009y\n                    <jats:sub>10<\/jats:sub>\n                    )]\n                    <jats:sup>3+<\/jats:sup>\n                    fragment ions also supported the (monomer\u2013[Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    )\n                    <jats:sup>4+<\/jats:sup>\n                    complex geometries of the [Tri\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    complex. The [(C-terminal)\u2013(C-terminal)] interaction geometry of the [Di\u2009+\u20093H]\n                    <jats:sup>3+<\/jats:sup>\n                    subunit is thought to be conserved in the [Tri\u2009+\u20094H]\n                    <jats:sup>4+<\/jats:sup>\n                    complex geometries.\n                  <\/jats:p>","DOI":"10.1177\/14690667241278462","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T03:40:24Z","timestamp":1725507624000},"page":"254-260","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of dimer and trimer complexes of the non-amyloidogenic rat islet amyloid polypeptide 21\u201337 by electrospray ionization-tandem mass spectrometry"],"prefix":"10.1177","volume":"30","author":[{"given":"Jin Young","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gumi, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seo Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gumi, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1541-3081","authenticated-orcid":false,"given":"Ho-Tae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gumi, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"bibr1-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-023-01082-1"},{"key":"bibr2-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1039\/D2CP01650H"},{"key":"bibr3-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00981"},{"key":"bibr4-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5036"},{"key":"bibr5-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1063\/1.4921073"},{"key":"bibr6-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.07.029"},{"key":"bibr7-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2005.10.009"},{"key":"bibr8-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00567"},{"key":"bibr9-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66602-9"},{"key":"bibr10-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/bi901751b"},{"key":"bibr11-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2010.01.006"},{"key":"bibr12-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/ja406831n"},{"key":"bibr13-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1293"},{"key":"bibr14-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1039\/C4CP05658B"},{"key":"bibr15-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/bi301525e"},{"key":"bibr16-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/ja903814q"},{"key":"bibr17-14690667241278462","doi-asserted-by":"publisher","DOI":"10.1021\/ja1081537"},{"key":"bibr18-14690667241278462","first-page":"179","volume":"12","author":"Kim 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