{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:27:14Z","timestamp":1777724834399,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p>\n                    Apoptosis of alveolar epithelial type II (AEC-II) cells induced by reactive oxygen species (ROS) contributes to extensive alveolar damage during acute lung injury. Hypercapnic acidosis and hypocapnic alkalosis are known to modulate ROSmediated lung damage. This study assessed the effects of acid\u2013base balance disturbances on hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    )-induced apoptosis of the AEC-II-like human cell line A549, which was cultured under different conditions of pH and CO\n                    <jats:sub>2<\/jats:sub>\n                    tension (normal pH and CO\n                    <jats:sub>2<\/jats:sub>\n                    , hypercapnic acidosis, metabolic acidosis, hypocapnic alkalosis and metabolic alkalosis). H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -induced apoptosis was assessed by a dye-uptake bioassay and induction of caspase activity, which were quantified using analytical digital photomicroscopy. Acidosis or alkalosis of the culture medium alone did not induce A549 cell apoptosis. Hypocapnic alkalosis significantly increased H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -induced apoptosis and caspase activation of A549 cells. Metabolic alkalosis non-significantly increased H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -induced A549 cell apoptosis and caspase activation. These data suggest that hypocapnic alkalosis intensifies oxidative-induced apoptosis of alveolar epithelial cells.\n                  <\/jats:p>","DOI":"10.1177\/147323000703500113","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T14:22:43Z","timestamp":1361974963000},"page":"118-126","source":"Crossref","is-referenced-by-count":10,"title":["Hypocapnic Alkalosis Enhances Oxidant-induced Apoptosis of Human Alveolar Epithelial Type II Cells"],"prefix":"10.1177","volume":"35","author":[{"given":"T","family":"Ando","sequence":"first","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, Faculty of Medical Sciences, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Mikawa","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, Faculty of Medical Sciences, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Nishina","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, Faculty of Medical Sciences, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Misumi","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, Faculty of Medical Sciences, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Obara","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, Faculty of Medical Sciences, Kobe University Graduate School of Medicine, Kobe, 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