{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T16:29:11Z","timestamp":1778344151079,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p>\n                    The routine diagnosis of hepatitis C virus (HCV) infection is based on the detection of anti-HCV antibodies by two main methods (enzyme immunoassay [EIA] and chemiluminescence immunoassay [CIA]) but false-positives are a problem. We investigated three anti-HCV tests: two CIAs (Cobas\u00ae e 601 and Architect\u00ae i2000SR); and one EIA (Ortho\u00ae HCV 3.0). Two other anti-HCV tests were also performed as supplementary and confirmatory tests, respectively: a recombinant strip immunoblot assay (RIBA HCV 3.0 SIA) and a reverse transcriptase polymerase chain reaction-based assay for HCV-RNA. After discriminating the false-positive results, the true anti-HCV seropositivity rate in 7156 serum samples was 0.91%. The seropositivity and false-positive rates for the Cobas\u00ae e 601, Architect\u00ae i2000SR and Ortho\u00ae HCV 3.0 anti-HCV tests were 1.9% and 0.99%, 1.2% and 0.29%, and 0.87% and 0.01%, respectively. The mean level of HCV-RNA was 3399 \u00d7 10\n                    <jats:sup>3<\/jats:sup>\n                    IU\/ml. Critical levels for false-positivity for HCV-RNA were a cut-off index of 200 for Cobas\u00ae e 601, a signal\/cut-off (S\/CO) of 5 for Architect\u00ae i2000SR and an S\/CO of 1.2 for Ortho\u00ae HCV 3.0. Positive and negative results for the RIBA HCV 3.0 SIA assay all accorded with the HCV-RNA assay, except for 23 (17%) \u2018indeterminate\u2019 results, all of which were negative with the HCV-RNA assay. In conclusion, to eliminate doubts related to false-positive findings in the initial HCV screening tests, additional confirmatory HCV-RNA assay should be performed.\n                  <\/jats:p>","DOI":"10.1177\/147323000903700516","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T14:22:43Z","timestamp":1361974963000},"page":"1420-1429","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation and Comparison of Three Different Anti-Hepatitis C Virus Antibody Tests based on Chemiluminescence and Enzyme-Linked Immunosorbent Assay Methods used in the Diagnosis of Hepatitis C Infections in Turkey"],"prefix":"10.1177","volume":"37","author":[{"given":"R","family":"Kesli","sequence":"first","affiliation":[{"name":"Department of Microbiology, Konya Education and Research Hospital, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Ozdemir","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Meram Faculty of Medicine, Selcuk University, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MG","family":"Kurtoglu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Konya Education and Research Hospital, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Baykan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Meram Faculty of Medicine, Selcuk University, Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Baysal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Meram Faculty of Medicine, Selcuk University, Konya, 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