{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T13:19:00Z","timestamp":1780319940331,"version":"3.54.1"},"reference-count":27,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>\n                    This study was designed to examine the expression of polo-like kinase 1 (PLK1) mRNA in 16 pterygia and 13 normal conjunctival tissue specimens using real-time fluorescent quantitative polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the housekeeping gene. The difference in threshold cycle value (\u0394 C\n                    <jats:sub>t<\/jats:sub>\n                    ) was derived for PLK1 and GAPDH for each sample assayed, and the difference between the paired samples (\u0394\u0394 C\n                    <jats:sub>t<\/jats:sub>\n                    ) was calculated. The mean \u00b1 SD \u0394 C\n                    <jats:sub>t<\/jats:sub>\n                    of PLK1 mRNA was 9.56 \u00b1 1.30 in pterygia compared with 10.71 \u00b1 1.39 in normal conjunctiva. The expression of PLK1 mRNA in pterygium was 2.08 - 2.36 times that in normal conjunctiva; this difference was statistically significant. Real-time fluorescent quantitative PCR analysis appears to be effective and sensitive when determining the level of PLK1 mRNA expression. Using this method, it was demonstrated that PLK1 mRNA is over-expressed in pterygia, indicating a probable role for PLK1 in their development.\n                  <\/jats:p>","DOI":"10.1177\/147323000903700621","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T14:22:43Z","timestamp":1361974963000},"page":"1851-1858","source":"Crossref","is-referenced-by-count":5,"title":["Real-Time Fluorescent Quantitative Polymerase Chain Reaction Study of Polo-Like Kinase-1 in Pterygia"],"prefix":"10.1177","volume":"37","author":[{"given":"H","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DH","family":"Lou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 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