{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:15:18Z","timestamp":1789917318856,"version":"4.0.1"},"reference-count":43,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T00:00:00Z","timestamp":1273795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diabetes and Vascular Disease Research"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>The aim of this study was to investigate the effects of acute, methionine-induced hyperhomocysteinaemia on endothelial function of skin microcirculation in diabetic compared with non-diabetic persons. Endothelial- and non-endothelial-dependent vasodilatation of the skin microcirculation were evaluated in 20 persons with type 2 diabetes (59.95\u00b12.36 years old) and 24 age- and gender-matched controls using laser Doppler flowmetry, during fasting and four hours after 0.1 g\/kg methionine-induced acute hyperhomocysteinaemia. Methionine loading caused a comparable increase of homocysteine in the two groups. Four hours after induction of acute hyperhomocysteinaemia, endothelial-dependent vasodilatation (acetylcholine effect) was lower in the diabetic patients compared with the controls ([mean\u00b1SEM] 8.09\u00b11.18-fold increase in diabetic patients vs. 11.11\u00b11.33-fold increase in controls, p=0.027). The same was observed for the endothelial-independent vasodilatation (sodium-nitroprusside effect, 7.55\u00b10.80-fold increase in the diabetic patients vs. 12.19\u00b11.26-fold increase in controls, p=0.008). Acute hyperhomocysteinaemia causes a decreased response of both endothelial-dependent and endothelial-independent vasodilatation of the skin microcirculation in diabetic compared with non-diabetic persons. These alterations might contribute to the vascular complications of hyperhomocysteinaemia in diabetes, and especially the microvascular ones.<\/jats:p>","DOI":"10.1177\/1479164110369687","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T20:40:08Z","timestamp":1273869608000},"page":"186-194","source":"Crossref","is-referenced-by-count":3,"title":["Acute hyperhomocysteinaemia blunts endothelial dependent and endothelial independent vasodilatation in diabetic patients"],"prefix":"10.1177","volume":"7","author":[{"given":"Ilias","family":"Chousos","sequence":"first","affiliation":[{"name":"First Department of Propaedeutic Medicine, University of Athens Medical School, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Despoina","family":"Perrea","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Surgery and Surgical Research 'Christeas Hall ', University of Athens Medical School, Athens, 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