{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:06:16Z","timestamp":1777435576491,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82360990"],"award-info":[{"award-number":["82360990"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      This study aimed to investigate the protective effects of 3\u2032-Methoxypuerarin (3\u2032-MOP) on myocardial ischemia\u2013reperfusion injury (MIRI) and elucidate its underlying mechanisms. Specifically, we examined its role in modulating N6-methyladenosine (m6A) methylation and suppressing cardiomyocyte pyroptosis in both\n                      <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                      , treatment with 3\u2032-MOP markedly reduced myocardial infarct size, preserved cardiac function, and alleviated histopathological injury following ischemia\/reperfusion. Consistently, 3\u2032-MOP suppressed m6A methylation and significantly decreased the expression of pyroptosis-related proteins, including NLRP3, cleaved GSDMD, cleaved Caspase-1, IL-1\u03b2, and IL-18.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      , 3\u2032-MOP decreased m6A methylation, destabilized NLRP3 mRNA, and inhibited pyroptosis in hypoxia\/reoxygenation-induced cardiomyocytes. Mechanistically, 3\u2032-MOP disrupted the interaction between insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) and NLRP3 mRNA, regulated m6A modification at predicted NLRP3 sites, and promoted mRNA degradation, thereby mimicking the effects of si-IGF2BP1 and attenuating pyroptottenuating pyroptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Innovation:<\/jats:title>\n                    <jats:p>\n                      3\u2032-MOP exerts cardioprotective effects against MIRI by modulating m6A methylation and inhibiting pyroptosis. This study is the first to demonstrate that 3\u2032-MOP regulates cardiomyocyte pyroptosis\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      the m6A\/IGF2BP1-NLRP3 axis, providing a novel epitranscriptomic mechanism for cardioprotection against MIRI.\n                      <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                      00, 000\u2013000.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15230864251389977","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T16:31:39Z","timestamp":1762187499000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["3\u2032-Methoxypuerarin Ameliorates Myocardial Ischemia\/Reperfusion-Induced Pyroptosis via Regulating IGF2BP1\/m6A\/NLRP3 Pathway"],"prefix":"10.1177","author":[{"given":"Haiyan","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sulail","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Neurobiology &amp; Biophysics, University of Washington, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deqin","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogenesis and Drug Research of Common Chronic Diseases in Guizhou Province, Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154407"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00022.2018"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154093"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-022-00735-4"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2022.07.005"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0045-z"},{"issue":"10","key":"e_1_3_4_8_1","first-page":"589","article-title":"[Study on treatment of infectious shock with recipe of liqi huoxue and kaibi gutuo]","volume":"15","author":"Jin MW","year":"1995","unstructured":"Jin MW, , Zhou ZY, , Zhang SW. 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