{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:21:26Z","timestamp":1789042886455,"version":"build-2803163510"},"reference-count":59,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82460114"],"award-info":[{"award-number":["82460114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foreign Cooperative Research Project of the Second Affiliated Hospital of Kunming Medical University","award":["2022dwhz09"],"award-info":[{"award-number":["2022dwhz09"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>Intestinal mucosal barrier injury is a major consequence of intestinal ischemia\u2013reperfusion (II\/R) and contributes to poor clinical outcomes. While mitophagy sustains mitochondrial homeostasis, how impaired autophagic flux disrupts intestinal barrier function remains unclear. We investigated the role of the deubiquitinase ubiquitin-specific protease 26 (USP26) and its downstream target syntaxin 17 (STX17) in regulating mitophagy and intestinal barrier repair.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      II\/R mouse models and oxygen\u2013glucose deprivation\/reperfusion (OGD\/R)-treated Caco-2 cells were used to evaluate intestinal barrier integrity. Functional manipulation, autophagy blockade, Co-IP, ubiquitination assays, Seahorse metabolic detection, and\n                      <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                      rescue assays were performed to dissect the USP26-STX17 regulatory axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      USP26 was markedly downregulated in II\/R intestinal tissues and OGD\/R Caco-2 cells, coinciding with impaired autophagy, mitochondrial dysfunction, and intestinal barrier disruption. USP26 overexpression preserved intestinal architecture, reduced permeability and apoptosis, and restored expression of the tight-junction proteins. Functionally, USP26 enhanced autophagic flux and mitophagy, as evidenced by increased LC3-II\/LC3-I ratios, reduced p62 accumulation, elevated PINK1\/Parkin signaling, improved adenosine triphosphate production, restored mitochondrial membrane potential, and normalized cellular bioenergetics; autophagy inhibition attenuated these protective phenotypes. Mechanistically, USP26 bound STX17 and stabilized it\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      deubiquitination. STX17 knockdown impaired autophagosome\u2013lysosome fusion and largely abolished USP26-mediated mitochondrial and intestinal barrier protection\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      This study identifies the USP26-STX17 axis as a previously unrecognized regulator of autophagosome\u2013lysosome fusion and mitophagy in II\/R injury. By restoring mitochondrial quality control and epithelial barrier integrity, USP26 emerges as a promising therapeutic target for the prevention and treatment of gut barrier dysfunction associated with critical illness.\n                      <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                      00, 000\u2013000.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15230864261484565","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:59:00Z","timestamp":1788796740000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["USP26 Promotes Autophagosome\u2013Lysosome Fusion to Improve Mitochondrial Function in the Repair of Gut Barrier Damage by Stabilizing STX17"],"prefix":"10.1177","author":[{"given":"Weiming","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pathology, Xiangya Hospital, School of Basic Medical Sciences, Central South University, Changsha, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0928-337X","authenticated-orcid":false,"given":"Wenliang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-020-00772-y"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00650-7"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102288"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9098145"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.08.010"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1087677"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2023.09.008"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1970.01340280030009"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.130025"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22083903"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2025.102177"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1154-0"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00058.2021"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28865"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-023-00916-x"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108552"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043219"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.08.047"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00904-x"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0299-4"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.854994"},{"issue":"1","key":"e_1_3_4_23_1","first-page":"32","article-title":"Mitochondrial quality control in human health and disease","volume":"11","author":"Liu BH","year":"2024","unstructured":"Liu BH, , Xu CZ, , Liu Y, et al. 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