{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T12:37:09Z","timestamp":1784810229827,"version":"3.55.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32260896"],"award-info":[{"award-number":["32260896"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Basic Research Projects of Yunnan Province","award":["202201AS070062"],"award-info":[{"award-number":["202201AS070062"]}]},{"name":"National Key Research and Development Program of China","award":["2022YFC2601603"],"award-info":[{"award-number":["2022YFC2601603"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Arboviruses, transmitted to humans and animals by blood-feeding arthropods, pose significant public health risks. This study investigates the types, distribution, and epidemiology of arboviruses in the central Yuxi region of Yunnan Province, providing crucial data for the prevention and control of mosquito-borne diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      In 2015, mosquitoes were collected from Tonghai County, Huaning County, Jiangchuan District, and Chengjiang County in Yuxi using light traps. The mosquitoes were homogenized, and virus isolation was performed by inoculating the homogenates onto BHK-21 (baby hamster kidney) and C6\/36 (\n                      <jats:italic toggle=\"yes\">Aedes albopictus<\/jats:italic>\n                      ) cell lines. Reverse Transcription Polymerase Chain Reaction (RT-PCR) amplification was performed with universal and virus-specific primers, followed by sequencing of positive products using bioinformatics tools (DNAstar, MEGA X).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 13,050 mosquitoes from four counties were collected, with 261 pools processed for virus detection. The mosquitoes belonged to three genera:\n                      <jats:italic toggle=\"yes\">Culex Anopheles<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Aedes<\/jats:italic>\n                      , with\n                      <jats:italic toggle=\"yes\">Culex tritaeniorhynchus<\/jats:italic>\n                      as the predominant species. Cytopathic effects were observed in 95 pools. Nine viruses were identified: Japanese Encephalitis Virus (JEV), Bannavirus, Getah Virus, Tibet Orbivirus (TIBOV), Nam Dinh Virus, Akabane Virus (AKAV), Ngewotan Virus (NWTV), Quang Binh Virus (QBV), and\n                      <jats:italic toggle=\"yes\">Culex pipiens pallens<\/jats:italic>\n                      densovirus. The distribution of viruses varied: six viruses with 35 isolates in Tonghai County; six viruses with 88 isolates in Huaning County; two viruses with five isolates in Chengjiang County; and no viruses were found in Jiangchuan District. TIBOV and NWTV were present in all three counties, while JEV, AKAV, and QBV were found only in Tonghai County.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Nine arboviruses were isolated across four counties in Yuxi, with varying distributions. This highlights the epidemiological diversity of mosquito-borne viruses in the region, emphasizing the need for targeted control measures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15303667251372145","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T11:26:29Z","timestamp":1762773989000},"page":"651-662","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and Identification of Arboviruses from Mosquitoes in Yuxi Region of Yunnan, China"],"prefix":"10.1177","volume":"25","author":[{"given":"Jue","family":"Ling","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Prevention and New Drug Development &amp; Yunnan Provincial Key Laboratory of Public Health and Biosafety, School of Public Health, Kunming Medical University, Kunming, China."},{"name":"Kunming Center for Disease Control and Prevention, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4085-3246","authenticated-orcid":false,"given":"Yantao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Prevention and New Drug Development &amp; Yunnan Provincial Key Laboratory of Public Health and Biosafety, School of Public Health, Kunming Medical University, Kunming, China."},{"name":"Kunming Medical University Haiyuan College, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"He","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunxiang","family":"Li","sequence":"additional","affiliation":[{"name":"Yuxi Emergency Center, Yuxi, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanzhi","family":"Xu","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Prevention and New Drug Development &amp; Yunnan Provincial Key Laboratory of Public Health and Biosafety, School of Public Health, Kunming Medical University, Kunming, China."},{"name":"Kunming Medical University Haiyuan College, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglin","family":"Wang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Cross-Border Infectious Disease Prevention and New Drug Development &amp; Yunnan Provincial Key Laboratory of Public Health and Biosafety, School of Public Health, Kunming Medical University, Kunming, China."},{"name":"Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.22194"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16081182"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0220753"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.984"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms1939.49.1003"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-002-0838-y"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-0934(98)00003-2"},{"issue":"3","key":"e_1_3_5_9_1","first-page":"135","article-title":"A survey of the relationships among the viruses not considered arboviruses, vertebrates, and arthropods","volume":"48","author":"Kuno G","year":"2004","unstructured":"Kuno G. 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