{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:44:48Z","timestamp":1790063088703,"version":"4.0.1"},"reference-count":26,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2027,2,4]],"date-time":"2027-02-04T00:00:00Z","timestamp":1801699200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.sagepub.com\/licence-information-for-chorus"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["2019-05380"],"award-info":[{"award-number":["2019-05380"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      There is limited understanding of the replication and transmission of bandaviruses and the influence of host genotype in successful infection. An\n                      <jats:italic toggle=\"yes\">in vitro Bandavirus<\/jats:italic>\n                      model, such as Lone Star virus (LSV,\n                      <jats:italic toggle=\"yes\">Bandavirus amblyommae<\/jats:italic>\n                      ), capable of propagating in standard cell lines, could provide some of this critical information. In this study, we sequenced the genome of LSV and profiled its relationship with a key host viral-interacting protein, Synaptogyrin-2 (SYNGR2), known to influence the replication of another\n                      <jats:italic toggle=\"yes\">Bandavirus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Bandavirus dabieense.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The genome of the LSV TMA 1381 strain was sequenced and assembled using Oxford Nanopore Technology. The expression of\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      was profiled and annotated in Vero cells.\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      knockout (KO) Vero clones were obtained\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      CRISPR-Cas9 gene editing of the first exon, present in all\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      isoforms. Following LSV infection, expression of\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      and LSV titer was measured in\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      -KO and wild-type cell lines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusions:<\/jats:title>\n                    <jats:p>\n                      Sequence variation and evidence of viral heterogeneity were detected across all segments of the LSV TMA 1381 strain (4 missense substitutions out of 7 single-nucleotide polymorphisms identified,\n                      <jats:italic toggle=\"yes\">q<\/jats:italic>\n                      &gt; 16). Important amino acid sequence differences for the nonstructural protein, known to directly interact with host SYNGR2, were observed between LSV and other bandaviruses (15.5\u201347.4%). The change in\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      expression in wild-type Vero cells was limited following LSV infection (1.77-fold). No difference in estimated LSV titer was detected between wild-type and\n                      <jats:italic toggle=\"yes\">SYNGR2<\/jats:italic>\n                      -KO Vero cells (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &gt; 0.16). Our data illustrate key distinctions from previous\n                      <jats:italic toggle=\"yes\">Bandavirus<\/jats:italic>\n                      reports and underline the need for future studies to explore the mechanisms of LSV replication and pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15303667261420983","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:57:49Z","timestamp":1770220669000},"page":"355-364","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Relationship Between Lone Star Virus and Synaptogyrin-2 Using Novel Viral and Host Models of Infections"],"prefix":"10.1177","volume":"26","author":[{"given":"Christian W.","family":"Eaton","sequence":"first","affiliation":[{"name":"Animal Science Department, University of Nebraska-Lincoln, Lincoln, Nebraska, USA."},{"name":"School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianna R.","family":"Walker","sequence":"additional","affiliation":[{"name":"Plant Genetics Research Unit, Agricultural Research Service, USDA, Columbia, Missouri, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiep L.X.","family":"Vu","sequence":"additional","affiliation":[{"name":"Animal Science Department, University of Nebraska-Lincoln, Lincoln, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Dustin","family":"Loy","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-1339","authenticated-orcid":false,"given":"Daniel C.","family":"Ciobanu","sequence":"additional","affiliation":[{"name":"Animal Science Department, University of Nebraska-Lincoln, Lincoln, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404851200"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1334224"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz715"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3102.240831"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02426-8"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.210641.116"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11092309"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1911.130792"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1969.18.789"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.801382"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2016.04.001"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2017.04.008"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2905.221488"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.715599"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062083"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit603"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-1108"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007750"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011029"},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1010095"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-016-3931-1"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15303667261420983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15303667261420983","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15303667261420983","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15303667261420983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T10:38:32Z","timestamp":1778927912000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15303667261420983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,4]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6,1]]}},"alternative-id":["10.1177\/15303667261420983"],"URL":"https:\/\/doi.org\/10.1177\/15303667261420983","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,4]]}}}