{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:03:33Z","timestamp":1784552613448,"version":"3.55.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne disease with a growing global distribution. In Armenia, 31 Ixodidae tick species have been identified, including\n                    <jats:italic toggle=\"yes\">Hyalomma<\/jats:italic>\n                    species. While CCHF virus (CCHFV) was previously isolated from ticks in the 1970s, no further studies were conducted until 2016, when antibody to CCHFV was identified.\n                  <\/jats:p>\n                  <jats:p>This study aimed to update data on the Ixodidae tick species, detect CCHFV in collected specimens, and perform molecular characterization of viral lineages circulating in Armenia during 2022\u20132024.<\/jats:p>\n                  <jats:p>Epizootiological investigations were performed across all 10 regions of Armenia. Fieldwork involved the collection of Ixodidae ticks, followed by morphological identification and testing of specimens. A total of 550 animals (including cattle, sheep, and horses) and surrounding open habitats were surveyed, resulting in the collection of 3158 Ixodidae ticks representing 12 species. These samples were organized into 960 pools and tested via reverse transcriptase polymerase chain reaction for CCHFV. Viral RNA was detected in 96 pools, all originating from ticks collected from cattle and representing 8 species. Positive samples were confined to two regions: Syunik and Tavush. Phylogenetic analysis revealed the presence of two CCHFV lineages in Armenia: Europe 1 (Subgroup V) and Europe 3 (Subgroup VII).<\/jats:p>\n                  <jats:p>Armenia\u2019s climatic and geographic diversity, expanding land use, population mobility, and growing tourism highlight the need for continued surveillance. The present identification of CCHFV in ticks in two regions emphasizes the importance of ongoing epizootiological monitoring and molecular characterization to assess the broader epidemic risk across the country.<\/jats:p>","DOI":"10.1177\/15303667261449543","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:45:23Z","timestamp":1777909523000},"page":"543-552","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Confirmation and Sequencing of Crimean-Congo Hemorrhagic Fever Virus in Ticks in Armenia (2022\u20132024)"],"prefix":"10.1177","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3351-3625","authenticated-orcid":false,"given":"Arsen","family":"Manucharyan","sequence":"first","affiliation":[{"name":"National Center for Disease Control and Prevention, Ministry of Health of the Republic of Armenia, Reference Laboratory Center, Yerevan, Armenia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayane","family":"Melik-Andreasyan","sequence":"additional","affiliation":[{"name":"National Center for Disease Control and Prevention, Ministry of Health of the Republic of Armenia, Reference Laboratory Center, Yerevan, Armenia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stepan","family":"Atoyan","sequence":"additional","affiliation":[{"name":"National Center for Disease Control and Prevention, Ministry of Health of the Republic of Armenia, Reference Laboratory Center, Yerevan, Armenia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Danielyan","sequence":"additional","affiliation":[{"name":"National Center for Disease Control and Prevention, Ministry of Health of the Republic of Armenia, Reference Laboratory Center, Yerevan, Armenia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1093817"},{"key":"e_1_3_5_3_1","unstructured":"Centers for Disease Control. 2024. About Crimean-Congo Hemorrhagic Fever. Available from: https:\/\/www.cdc.gov\/crimean-congo-hemorrhagic\/about\/index.html [Last accessed: April24 2025]."},{"issue":"2","key":"e_1_3_5_4_1","first-page":"127","article-title":"Genetic diversity of Crimean Congo Hemorrhagic Fever virus strains from Iran","volume":"10","author":"Chinikar S","year":"2016","unstructured":"Chinikar S, , Bouzari S, , Shokrgozar MA, et al. Genetic diversity of Crimean Congo Hemorrhagic Fever virus strains from Iran. J Arthropod Borne Dis 2016;10(2):127\u2013140.","journal-title":"J Arthropod Borne Dis"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.10.014"},{"key":"e_1_3_5_6_1","volume-title":"Conference of Research Workers in Animal Diseases","author":"Danelyan A","year":"2025","unstructured":"Danelyan A, , Tumanyan PG, , Hovhannisyan AA. 2025. Crimean-Congo hemorrhagic fever seropositive cases among livestock in the Tavush Region of Armenia, Conference of Research Workers in Animal Diseases. Chicago, IL, USA."},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/hygiene5020018"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-024-00970-5"},{"issue":"1","key":"e_1_3_5_9_1","first-page":"81","article-title":"Arthropods (Arthropoda) of Armenia of medical importance, their biological and ecological peculiarities","volume":"6","author":"Dilbaryan K","year":"2016","unstructured":"Dilbaryan K, , Hovhannisyan V. Arthropods (Arthropoda) of Armenia of medical importance, their biological and ecological peculiarities. Med. Sci. Armen 2016;6(1):81\u201389.","journal-title":"Med. Sci. Armen"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.4274\/tpd.galenos.2020.6985"},{"key":"e_1_3_5_11_1","unstructured":"Filippova N. 1966. Argasid ticks (Argasidae)."},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-9062.126410"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.67.397"},{"issue":"1","key":"e_1_3_5_14_1","first-page":"9","article-title":"Evidence of Crimean-Congo Haemorrhagic fever virus occurrence in Ixodidae Ticks of Armenia","volume":"13","author":"Gevorgyan H","year":"2019","unstructured":"Gevorgyan H, , Grigoryan GG, , Atoyan HA, et al. Evidence of Crimean-Congo Haemorrhagic fever virus occurrence in Ixodidae Ticks of Armenia. JAD 2019;13(1):9\u201316.","journal-title":"JAD"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0923-2516(99)80013-2"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158049"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1008.030928"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.52340\/9789941869020"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0460"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2015.12.003"},{"key":"e_1_3_5_22_1","first-page":"173","article-title":"Antibodies to CHF virus in human and cattle blood sera from Armenia SSR (NAMRU-T939)","volume":"22","author":"Matevosyan K","year":"1974","unstructured":"Matevosyan K, , Semashko I, , Rubin S, , Chumakov M. Antibodies to CHF virus in human and cattle blood sera from Armenia SSR (NAMRU-T939). Tr Inst Polio Virus Entsef Akad Med Nauk SSSR 1974;22:173\u2013175.","journal-title":"Tr Inst Polio Virus Entsef Akad Med Nauk SSSR"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects14090771"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0509"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110061"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2025.102806"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.04.017"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.08.029"},{"key":"e_1_3_5_29_1","unstructured":"Public Health Scotland. 2022. Crimean-Congo haemorrhagic fever in Georgia. 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Results from the 1972\u20131974 works on isolation and investigation of CHF-Congo Dhori and Bhanja viruses in Azerbaijan and Armenia. Academy of Medical Sciences. Moscow USSR pp. 354\u2013355."},{"key":"e_1_3_5_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9091907"},{"key":"e_1_3_5_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003168"},{"key":"e_1_3_5_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-025-10728-6"},{"key":"e_1_3_5_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365540903362501"},{"key":"e_1_3_5_37_1","doi-asserted-by":"publisher","DOI":"10.1501\/Vetfak_0000002604"},{"key":"e_1_3_5_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0266177"},{"key":"e_1_3_5_39_1","unstructured":"World Health Organization. 2025. Crimean-Congo haemorrhagic fever. 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