{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T11:34:21Z","timestamp":1786361661289,"version":"build-2736575974"},"reference-count":43,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI171014"],"award-info":[{"award-number":["R01AI171014"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI183477"],"award-info":[{"award-number":["R21AI183477"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"\n \n Background:<\/jats:italic>\n <\/jats:title>\n \n Cockroaches, including the German cockroach (\n Blattella germanica<\/jats:italic>\n ) can harbor and disseminate enteric human pathogens, such as\n Salmonella<\/jats:italic>\n spp. Detecting\n Salmonella<\/jats:italic>\n in field-collected cockroach samples is important for source attribution and monitoring public health risk, but is complicated by the low abundance of the pathogen relative to the rich and complex cockroach microbiota. Studies using different culture methods to detect\n Salmonella<\/jats:italic>\n in cockroaches have reported disparate results, emphasizing the need for sensitive, accurate strategies to recover and identify\n Salmonella<\/jats:italic>\n from infected cockroaches.\n <\/jats:p>\n <\/jats:sec>\n \n \n Materials and Methods:<\/jats:italic>\n <\/jats:title>\n \n Here, we evaluated the efficacy of several\n Salmonella<\/jats:italic>\n enrichment and selective detection strategies\u2014Rappaport\u2013Vassiliadis (RV) broth enrichment, Tetrathionate (TT) broth enrichment, and a sequential TT followed by RV enrichment, combined with selective plating on Brilliant Green (BG) or Xylose Lysine Tergitol 4 (XLT-4) agar. Efficacy was assessed on German cockroach samples collected 1\u20137 days after experimental infection with a laboratory reference strain of\n Salmonella.<\/jats:italic>\n The relative abundance of\n Salmonella<\/jats:italic>\n in each enrichment broth was determined by 16S rRNA gene amplicon sequencing, and colony morphologies on selective plates were visually compared.\n <\/jats:p>\n <\/jats:sec>\n \n \n Results:<\/jats:italic>\n <\/jats:title>\n \n Overall, RV broth, TT broth, and sequential TT-RV broth strategies were equally effective at enriching\n Salmonella<\/jats:italic>\n from infected cockroach samples. However, selective plating on XLT-4 was more effective at differentiating\n Salmonella<\/jats:italic>\n from commensal\n Pseudomonas<\/jats:italic>\n and\n Serratia<\/jats:italic>\n spp. that were also enriched in RV and TT broth and produced similar colony phenotypes to\n Salmonella<\/jats:italic>\n on BG agar.\n <\/jats:p>\n <\/jats:sec>\n \n \n Conclusions:<\/jats:italic>\n <\/jats:title>\n \n These results enhance methodological capacity for\n Salmonella<\/jats:italic>\n surveillance in cockroaches and highlight the importance of considering the potential for cockroach commensals to produce false positives, interfering with accurate pathogen detection.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/15303667261469043","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:09:17Z","timestamp":1784214557000},"page":"600-607","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of Enrichment and Selective Culture Strategies for Detecting\n Salmonella<\/i>\n from Cockroaches"],"prefix":"10.1177","volume":"26","author":[{"given":"Lipika","family":"Das","sequence":"first","affiliation":[{"name":"Department of Entomology, Center for Urban and Industrial Pest Management, Purdue University, West Lafayette, Indiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya B.","family":"Slizovskiy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, Indiana, USA."},{"name":"Regenstrief Center for Healthcare Engineering, Purdue University, West Lafayette, Indiana, USA."},{"name":"Purdue Applied Microbiome Sciences Program, Purdue University, West Lafayette, Indiana, USA."},{"name":"Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, Indiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7607-3220","authenticated-orcid":false,"given":"Jose E.","family":"Pietri","sequence":"additional","affiliation":[{"name":"Department of Entomology, Center for Urban and Industrial Pest Management, Purdue University, West Lafayette, Indiana, USA."},{"name":"Purdue Applied Microbiome Sciences Program, Purdue University, West Lafayette, Indiana, USA."},{"name":"Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, Indiana, USA."},{"name":"Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00408-25"},{"key":"e_1_3_4_3_1","unstructured":"Andrews S Segonds-Pichon FK Biggins A et al. 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