{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:03:39Z","timestamp":1789776219480,"version":"4.0.1"},"reference-count":41,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Urinary tract infections (UTIs) in cats are increasingly recognized as clinically relevant conditions frequently associated with multidrug-resistant (MDR) bacteria of potential zoonotic origin, yet genomic data on feline uropathogens remain scarce in Tunisia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      We used whole-genome sequencing to characterize seven bacterial isolates recovered from six cats with clinical signs of UTI: Mammaliicoccus lentus (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 2), Staphylococcus schleiferi (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1), Mammaliicoccus sciuri (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1), Enterococcus faecalis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1), Enterococcus casseliflavus (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1), and Klebsiella aerogenes (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 1).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Resistome analysis revealed determinants conferring resistance to \u03b2-lactams (blaZ, blaCMY-132), methicillin (mecC-type), macrolides (erm(43), ermB), tetracyclines (tet(M), tet(45), tetB), fosfomycins (fosI, fosB, fosA5), and aminoglycosides (aac(6\u2032), aph(3\u2032)-IIIa, aph(6)-Id), alongside efflux pump genes (efrA, sepA, sdrM, oqxA, KpnE\/F\/G), vancomycin-operon genes (vanT, vanY, vanC, vanG), and biofilm\/biocide-tolerance genes (salB, qacG). Notably, M. lentus S104 carried mecC-type elements, the first such report in Tunisia, while K. aerogenes displayed an extensive MDR profile, including blaCMY-132 and fosA5. Multilocus sequence typing\/ribosomal multilocus sequence typing (MLST\/rMLST) identified diverse lineages, including the internationally distributed E. faecalis ST19 and the rarely reported K. aerogenes ST242. Plasmids were absent in all isolates; a Tn916\/1545-type transposon occurred in E. casseliflavus, and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems were unevenly distributed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These findings highlight companion animals as reservoirs of clinically important resistance genes, reinforcing the need for One Health AMR surveillance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15303667261477573","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:28:22Z","timestamp":1786627702000},"page":"717-727","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole-Genome Sequencing of Feline Uropathogens Reveals Multidrug Resistance and Zoonotic Potential in Domestic Cats in Tunisia"],"prefix":"10.1177","volume":"26","author":[{"given":"Hajer","family":"Kilani","sequence":"first","affiliation":[{"name":"Laboratory of Antibiotic Resistance LR99ES09, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia."},{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis, Tunisia."},{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, Ariana, Tunisia."},{"name":"Department of Microbiology, Hospital of Charles Nicolle, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaineb","family":"Hamzaoui","sequence":"additional","affiliation":[{"name":"Laboratory of Antibiotic Resistance LR99ES09, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia."},{"name":"Department of Microbiology, Hospital of Charles Nicolle, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Ferjani","sequence":"additional","affiliation":[{"name":"Laboratory of Antibiotic Resistance LR99ES09, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia."},{"name":"Department of Microbiology, Hospital of Charles Nicolle, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Lengliz","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghassan","family":"Tayh","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, Ariana, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Ben Zedira","sequence":"additional","affiliation":[{"name":"Laboratory of Electro-Mechanic Systems (LASEM), National School of Engineers of Sfax (ENIS), University of Sfax, B.P. 1173, km 3.5 Soukra, 3038 Sfax, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabrouk","family":"Mosbahi","sequence":"additional","affiliation":[{"name":"Higher Institute of Applied Sciences and Technology of Gafsa, University of Gafsa, Gafsa 2112, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamia","family":"Kanzari","sequence":"additional","affiliation":[{"name":"Laboratory of Antibiotic Resistance LR99ES09, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia."},{"name":"Department of Microbiology, Hospital of Charles Nicolle, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faten","family":"Ben Chehida","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National School of Veterinary Medicine, University of Manouba, Ariana, 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