{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:42:09Z","timestamp":1792672929077,"version":"4.5.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"University Malaya Research Fund Assistance-Early Career Research Grant","award":["BKP-ECRG-097"],"award-info":[{"award-number":["BKP-ECRG-097"]}]},{"name":"Ministry of Higher Education of Malaysia","award":["PR002-2024 (PRGS\/1\/2024\/SKK13\/UM\/01\/1)"],"award-info":[{"award-number":["PR002-2024 (PRGS\/1\/2024\/SKK13\/UM\/01\/1)"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2026,12,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Zoonotic malaria caused by simian\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      species presents increasing diagnostic challenges in endemic regions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      This study developed and evaluated malachite green-based loop-mediated isothermal amplification (MG-LAMP) assays for detecting five zoonotic malaria parasites (\n                      <jats:italic toggle=\"yes\">P. knowlesi, P. cynomolgi, P. inui, P. coatneyi<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">P. fieldi<\/jats:italic>\n                      ). The assays enable rapid visual detection through a colorimetric change without requiring specialized equipment. To reduce carryover contamination, deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) were incorporated into the reaction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result:<\/jats:title>\n                    <jats:p>\n                      All five UDG MG-LAMP assays demonstrated high analytical sensitivity, with limits of detection of 1 copy\/\u00b5L\n                      <jats:italic toggle=\"yes\">for P. cynomolgi, P. coatneyi<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">P. fieldi<\/jats:italic>\n                      , and 10 copies\/\u00b5L for\n                      <jats:italic toggle=\"yes\">P. knowlesi<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">P. inui<\/jats:italic>\n                      . The assays showed 100% specificity, with no cross-reactivity among target species. Malachite green enabled clear visual discrimination between positive and negative reactions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>A simplified direct blood lysis method requiring less than 20 min was also developed to enable rapid DNA preparation without column-based purification. Overall, the MG-LAMP assays combined with UDG and rapid lysis provide a sensitive, specific, and practical approach for diagnosing zoonotic malaria, particularly in resource-limited endemic settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15303667261484456","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T13:12:24Z","timestamp":1788181944000},"page":"780-785","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Uracil-DNA Glycosylase-Enhanced Loop-Mediated Isothermal Amplification Assays for Detection of Zoonotic Malaria\n                    <i>Plasmodium<\/i>\n                    Parasites"],"prefix":"10.1177","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4544-695X","authenticated-orcid":false,"given":"Meng Yee","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Lutfi","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Ex-Situ Conservation Division, Department of Wildlife, National Wildlife Forensic Laboratory, National Parks Peninsular Malaysia, Kuala Lumpur, Malaysia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S003118202300077X"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006922"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit184"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4cc00540f"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00811-09"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2016.17.3.421"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107249"},{"issue":"4","key":"e_1_3_2_10_1","first-page":"765","article-title":"Loop-mediated isothermal amplification for diagnosis of zoonotic malaria","volume":"111","author":"Lai MY","year":"2024","unstructured":"Lai MY, , Sohairi AN, , Zen LPY, et al. Loop-mediated isothermal amplification for diagnosis of zoonotic malaria. Am J Trop Med Hyg 2024a;111(4):765\u2013769.","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0569"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-8-73"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002015"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151437"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90181-8"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-009-0669-9"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1214-1219.2004"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5636-5643.2004"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115628"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.57"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107280"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03615-13"},{"key":"e_1_3_2_25_1","unstructured":"World Health Organization (WHO). World malaria report 2024: Addressing inequity in the global malaria response. WHO: Geneva Switzerland; 2025. Available from: https:\/\/www.who.int\/teams\/global-malaria-programme\/reports\/world-malaria-report-2025 [Last accessed: January6 2026]."},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14070651"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2708.204502"},{"key":"e_1_3_2_28_1","doi-asserted-by":"publisher","DOI":"10.47665\/tb.37.4.1124"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.2144\/btn-2020-0078"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15303667261484456","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15303667261484456","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15303667261484456","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:46:30Z","timestamp":1792669590000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15303667261484456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":28,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,12,1]]}},"alternative-id":["10.1177\/15303667261484456"],"URL":"https:\/\/doi.org\/10.1177\/15303667261484456","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]}}}