{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:28:02Z","timestamp":1777940882246,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      To construct plasmids with Hre\n                      <jats:sub>2<\/jats:sub>\n                      .Grp78 chimeric promoter regulating fusion gene TK\/VP3 and elaborate the effects of overexpressed TK\/VP3 on nasopharyngeal carcinoma cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Four plasmids were constructed, including pcDNA3.1-CMV-TK\/VP3, pcDNA3.1-Hre\n                      <jats:sub>2<\/jats:sub>\n                      .TK\/VP3, pcDNA3.1-Grp78.TK\/VP3, and pcDNA3.1-Hre\n                      <jats:sub>2<\/jats:sub>\n                      .Grp78.TK\/VP3. The human nasopharyngeal carcinoma cell line HNE1 cells were transfected with the 4 plasmids, respectively. Cell viabilities were evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and apoptosis was conducted using flow cytometry analysis. The expression of TK, VP3, Grp78, and hypoxia-inducible factor 1\u03b1 and apoptosis-related proteins was determined by real-time quantitative polymerase chain reaction and Western blotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The recombinant plasmids that could steadily overexpress TK and VP3 were successfully constructed. Expression of TK and VP3 in cells transfected with pcDNA3.1-Hre\n                      <jats:sub>2<\/jats:sub>\n                      .TK\/VP3 and pcDNA3.1-Grp78.TK\/VP3 was significantly higher than pcDNA3.1-CMV-TK\/VP3, and expression in cells transfected with pcDNA3.1-Hre\n                      <jats:sub>2<\/jats:sub>\n                      .Grp78.TK\/VP3 was the highest. Under glucose deprivation or hypoxia condition, Grp78 or hypoxia-inducible factor 1\u03b1 was overexpressed so that expression of TK and VP3 was significantly upregulated, which could further inhibit cell proliferation and enhance cell apoptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      We successfully constructed 4 plasmids with Hre\n                      <jats:sub>2<\/jats:sub>\n                      .Grp78 chimeric promoter regulating fusion gene TK\/VP3, which could significantly inhibit the proliferation as well as enhance the apoptosis of nasopharyngeal carcinoma cells under glucose deprivation or hypoxia condition.\n                    <\/jats:p>\n                  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