{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:28:47Z","timestamp":1777940927980,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011710","name":"Shaanxi Provincial Department of Science and Technology","doi-asserted-by":"crossref","award":["NO.2016SF-234, NO.2018KJXX-093"],"award-info":[{"award-number":["NO.2016SF-234, NO.2018KJXX-093"]}],"id":[{"id":"10.13039\/501100011710","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:p>Although the treatment of liver cancer has made great progress, the mechanism of its occurrence is not completely clear. miR-155 plays an important regulatory role in tumorigenesis and development, including survival, proliferation, migration and invasion. However, the role and regulatory mechanism of miR-155 in liver cancer has rarely been reported. We analyzed miR-155 expression in liver cancer tissue samples and cell lines by qRT-PCR. The expression of miR-155 was measured by qRT-PCR before and after miR-155-mimic and sh-miR-155 transfection. CCK-8 and clonogenic assays were used to detect the proliferation of liver cancer cells. Cell scratch and invasion assays were used to detect migration and invasion. RNA-seq was used to detect the difference in RNA expression in liver cancer cells. SRPK1 expression was detected in liver cancer cells before and after transfection by qRT-PCR and western blotting. We observed that miR-155 was downregulated in liver cancer tissues compared with normal tissues. Furthermore, we demonstrated that liver cancer cell proliferation, migration and invasion are markedly suppressed by miR-155. Importantly, we also demonstrated that SRPK1 is directly regulated by miR-155 during the process of liver cancer cell proliferation and metastasis. Finally, the overexpression of miR-155 inhibits malignant biological behavior of human liver cancer cells. We report the abnormal expression of the miR-155 cluster in liver cancer cells, which inhibits cancer cell proliferation and metastasis. In addition, we identified SRPK1 as a target gene of miR-155 during the process of liver cancer cell proliferation and metastasis.<\/jats:p>","DOI":"10.1177\/1533033820957021","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T03:44:42Z","timestamp":1617853482000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["MiR-155 Inhibits Malignant Biological Behavior of Human Liver Cancer Cells by Regulating SRPK1"],"prefix":"10.1177","volume":"20","author":[{"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery, The Affiliated Hospital of Shananxi University of Chinese Medicine, Xianyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-tai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The Affiliated Hospital of Shananxi University of Chinese Medicine, Xianyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3875-7012","authenticated-orcid":false,"given":"Wei-hong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The Affiliated Hospital of Shananxi University of Chinese Medicine, Xianyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"issue":"13","key":"bibr1-1533033820957021","first-page":"950","volume":"25","author":"Ndom P","year":"2019","journal-title":"Ecancermedicalscience"},{"issue":"13","key":"bibr2-1533033820957021","first-page":"109274","volume":"118","author":"Ranganathan S","year":"2019","journal-title":"Pediatr Dev Pathol"},{"issue":"10","key":"bibr3-1533033820957021","first-page":"949","volume":"13","author":"Hainaut P","year":"2019","journal-title":"Ecancermedicalscience"},{"key":"bibr4-1533033820957021","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2019.20.9.2723"},{"issue":"18","key":"bibr5-1533033820957021","first-page":"1939","volume":"23","author":"Wang Y","year":"2019","journal-title":"Technol Cancer Res Treat"},{"key":"bibr6-1533033820957021","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.919219"},{"issue":"3","key":"bibr7-1533033820957021","first-page":"3381","volume":"18","author":"Ning S","year":"2019","journal-title":"Oncol Lett"},{"issue":"13","key":"bibr8-1533033820957021","first-page":"1245","volume":"25","author":"Shi Q","year":"2019","journal-title":"J Med Virol"},{"key":"bibr9-1533033820957021","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25866"},{"issue":"13","key":"bibr10-1533033820957021","first-page":"5682","volume":"23","author":"Dong ZC","year":"2019","journal-title":"Eur Rev Med Pharmacol Sci"},{"issue":"12","key":"bibr11-1533033820957021","first-page":"893","volume":"35","author":"Huang Y-Q","year":"2019","journal-title":"J Hum Hypertens"},{"issue":"1","key":"bibr12-1533033820957021","first-page":"1617","volume":"18","author":"Li ZR","year":"2019","journal-title":"Oncol Lett"},{"key":"bibr13-1533033820957021","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.03.078"},{"issue":"1","key":"bibr14-1533033820957021","first-page":"1487","volume":"12","author":"Bowler E","year":"2018","journal-title":"BMC Cancer"},{"issue":"10","key":"bibr15-1533033820957021","first-page":"3356","volume":"23","author":"Mielgo A","year":"2019","journal-title":"Cold Spring Harb Perspect Med"},{"key":"bibr16-1533033820957021","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.2350"},{"issue":"15","key":"bibr17-1533033820957021","first-page":"377","volume":"34","author":"Xiao T","year":"2019","journal-title":"Toxicol Appl Pharmacol"},{"key":"bibr18-1533033820957021","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900386"},{"key":"bibr19-1533033820957021","doi-asserted-by":"publisher","DOI":"10.1016\/j.aohep.2018.11.007"},{"issue":"4","key":"bibr20-1533033820957021","first-page":"642","volume":"11","author":"Cuadros M","year":"2019","journal-title":"Clin Transl Oncol"}],"container-title":["Technology in Cancer Research &amp; 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