{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:30:45Z","timestamp":1777941045905,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Nanjing Technological Development Program","award":["201605049"],"award-info":[{"award-number":["201605049"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To uncover potential diagnostic biomarkers for endocervical adenocarcinoma (EAC) and adenocarcinoma in situ (AIS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental design:<\/jats:title>\n                    <jats:p>Quantitative label-free liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS\/MS) peptidomics strategies were employed to profile 8 cervical mucus (CM) samples, including 3 EAC cases, 2 AIS cases and 3 normal controls (Ctrl).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Among the 3721 exclusive peptides identified, 12 (5 up-regulated and 7 down-regulated) endogenous peptides were significantly expressed in EAC compared to healthy controls (EAC\/Ctrl); 10 (7 up-regulated and 3 down-regulated) endogenous peptides were significantly expressed in AIS compared to healthy controls (AIS\/Ctrl); 11 (6 up-regulated and 5 down-regulated) endogenous peptides were significantly expressed in EAC compared to AIS (EAC\/AIS) (absolute fold change \u22651.5, Benjamini-Hochberg adjusted p-value\u2009\u22640.05). Among these identifications, annexin A1 (ANXA1) was found to be down-regulated both in EAC and AIS, and its unique peptide (FIENEEQEYVQTVK) may be promising indicators for cervical glandular epithelial lesions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>This is the first study to utilize CM peptidomics in cervical glandular malignancies, which may reveal the novel noninvasive biomarkers for EAC and AIS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1533033821997825","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T04:46:03Z","timestamp":1613969163000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ"],"prefix":"10.1177","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7602-7440","authenticated-orcid":false,"given":"Feng","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Pathology, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Radiology, Nanjing Children\u2019s Hospital Affiliated to Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhifang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pathology, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianying","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2783-2929","authenticated-orcid":false,"given":"Yun","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"bibr1-1533033821997825","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"bibr2-1533033821997825","unstructured":"Kurman RJ, Carcangiu ML, Herrington S, et al. WHO Classification of Tumours of Female Reproductive Organs. IARC Press; 2014:183\u2013194."},{"key":"bibr3-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980209)75:4<536::AID-IJC8>3.0.CO;2-U"},{"issue":"4","key":"bibr4-1533033821997825","first-page":"645","volume":"10","author":"Mathew A","year":"2009","journal-title":"Asian Pac J Cancer Prev"},{"key":"bibr5-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1089\/jwh.2011.3385"},{"key":"bibr6-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.971"},{"key":"bibr7-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32470-X"},{"key":"bibr8-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1999.5364"},{"key":"bibr9-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20877"},{"key":"bibr10-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2012.01.034"},{"key":"bibr11-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-017-9142-4"},{"key":"bibr12-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4374"},{"key":"bibr13-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-017-4587-2"},{"key":"bibr14-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106488"},{"key":"bibr15-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.211714"},{"key":"bibr16-1533033821997825","doi-asserted-by":"publisher","DOI":"10.2174\/1389203717666160724203746"},{"key":"bibr17-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(06)70584-8"},{"key":"bibr18-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1186\/s12953-014-0049-y"},{"key":"bibr19-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2016.09.014"},{"issue":"7","key":"bibr20-1533033821997825","first-page":"151","volume":"7","author":"Eze JN","year":"2013","journal-title":"Clin Med Insights Oncol"},{"key":"bibr21-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-11074-5"},{"key":"bibr22-1533033821997825","doi-asserted-by":"publisher","DOI":"10.5858\/134.3.331"},{"issue":"6","key":"bibr23-1533033821997825","first-page":"523","volume":"120","author":"Jones BA","year":"1996","journal-title":"Arch Pathol Lab Med"},{"key":"bibr24-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21724"},{"key":"bibr25-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1698772"},{"key":"bibr26-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1186\/s13027-019-0250-9"},{"key":"bibr27-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000408"},{"key":"bibr28-1533033821997825","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001224"},{"key":"bibr29-1533033821997825","doi-asserted-by":"publisher","DOI":"10.2217\/fon.13.114"}],"container-title":["Technology in Cancer Research &amp; Treatment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1533033821997825","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1533033821997825","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1533033821997825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:09:58Z","timestamp":1777702198000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1533033821997825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,1]]},"references-count":29,"alternative-id":["10.1177\/1533033821997825"],"URL":"https:\/\/doi.org\/10.1177\/1533033821997825","relation":{},"ISSN":["1533-0346","1533-0338"],"issn-type":[{"value":"1533-0346","type":"print"},{"value":"1533-0338","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,1]]},"article-number":"1533033821997825"}}