{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:44:17Z","timestamp":1791233057105,"version":"4.1.0"},"reference-count":18,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81971088"],"award-info":[{"award-number":["81971088"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2017MH074"],"award-info":[{"award-number":["ZR2017MH074"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2018MC008"],"award-info":[{"award-number":["ZR2018MC008"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation Project of Shandong Academy of Medical Sciences"},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2014M550370"],"award-info":[{"award-number":["2014M550370"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2015T80740"],"award-info":[{"award-number":["2015T80740"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Academic promotion program of Shandong First Medical University","award":["2019QL024"],"award-info":[{"award-number":["2019QL024"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Malignant melanoma is a common malignant tumor and one of the tumors with the fastest growing incidence. The effect of microRNAs on the biological processing of malignant melanoma cells also have been reported. This study explores the ability of miR-498 to regulate the progression of malignant melanoma cells.\n                    <jats:bold>Methods:<\/jats:bold>\n                    The expression of miR-498 was detected by RT-qPCR. The proliferation, invasion, and migration of malignant melanoma cells were measured by cell counting kit-8, clone formation, and transwell assays. Flow cytometry assay detected the percentage of apoptotic cells. Western blot was used to detect the expression of markers related to epithelial\u2013mesenchymal transition. The correction of miR-498 and UBE2T was explored by dual-luciferase assay and Western blot.\n                    <jats:bold>Results:<\/jats:bold>\n                    Overexpression of miR-498 inhibited the proliferation, invasion, migration, and induced cell apoptosis of M14 and A375 cells. In addition, the expression of epithelial\u2013mesenchymal transition-related factors was altered by the overexpression of miR-498. miR-498 can directly target UBE2T 3'-UTR and inhibit UBE2T protein expression. The overexpression of UBE2T reversed the inhibitory effects of miR-498 on the progression of malignant melanoma cells. Furthermore, UBE2T mRNA was significantly highly expressed in malignant melanoma tissues. The high expression of UBE2T was associated with the poor overall survival rate of malignant melanoma patients.\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Altogether, our findings demonstrated that miR-498 significantly inhibited the proliferation, invasion, migration, and induced apoptosis of malignant melanoma cells and confirmed that miR-498 regulated malignant melanoma cell progression by targeting UBE2T.\n                  <\/jats:p>","DOI":"10.1177\/15330338221082431","type":"journal-article","created":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T05:12:11Z","timestamp":1646370731000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["miR-498 Targets UBE2T to Inhibit the Proliferation of Malignant Melanoma Cells"],"prefix":"10.1177","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9784-563X","authenticated-orcid":false,"given":"Wen","family":"Cao","sequence":"first","affiliation":[{"name":"Health College, Yantai Nanshan University, Yantai, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin","family":"Li","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Shandong First Medical University &amp; Shandong Academy of Medical Sciences, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Xia","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7415-2352","authenticated-orcid":false,"given":"Shu-Hong","family":"Huang","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Shandong First Medical University &amp; Shandong Academy of Medical Sciences, Shandong, China"},{"name":"Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning-Ning","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/dth.12522"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1442-2050.2001.00193.x"},{"issue":"2","key":"e_1_3_2_4_2","first-page":"356","article-title":"Malignant melanoma current status","volume":"77","author":"Winkler JK","year":"2017","unstructured":"Winkler JK, Buderbakhaya K, Dimitrakopouloustrauss A. Malignant melanoma current status. Radiologe. 2017;77(2):356.","journal-title":"Radiologe"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.63"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7705"},{"issue":"24","key":"e_1_3_2_7_2","first-page":"4762","article-title":"Low miR-498 expression levels are associated with poor prognosis in ovarian cancer","volume":"19","author":"Cong J","year":"2016","unstructured":"Cong J, Liu R, Wang X, et al. Low miR-498 expression levels are associated with poor prognosis in ovarian cancer. 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