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Increasing evidence has demonstrated that \u03b2-tubulin 4A (TUBB4A) plays a key role in the development and progression of several types of human cancer. However, the potential function of TUBB4A in cutaneous melanoma remains to be determined. Methods: We first performed a differential expression analysis based on skin melanoma tissues and normal tissues from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) datasets and then a survival analysis to identify prognostic-related key genes. We further conducted in vitro biochemical experiments to verify the functional roles of the key gene TUBB4A. Two small-molecule inhibitors of TUBB4A, Dihydroartemisinin (DHA) and Nocodazole, were used to validate the effect of TUBB4A on the apoptosis and cell cycle of melanoma cells. Results: We found that TUBB4A expression was positively correlated to the overall survival (OS) of cutaneous melanoma patients. The coexpressed genes with TUBB4A were enriched in melanoma-related pathways and functions. The experimental results showed that knockdown of TUBB4A inhibited the proliferation and migration of A375 and B16-F10 melanoma cells. Moreover, DHA and Nocodazole promoted the apoptosis of melanoma cells and blocked the melanoma tumor cell cycle in the G2\/M stage. Conclusion: TUBB4A may be a prognostic biomarker and therapeutic target for melanoma.<\/jats:p>","DOI":"10.1177\/15330338231184842","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T02:19:07Z","timestamp":1689214747000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification of TUBB4A as a Prognostic Biomarker of Melanoma by Transcriptomic Data and\n                    <i>In Vitro<\/i>\n                    Experiments"],"prefix":"10.1177","volume":"22","author":[{"given":"Jiaqi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Dermatology, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, China"},{"name":"Department of Radiotherapy, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, 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