{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:03:04Z","timestamp":1789862584351,"version":"4.0.1"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Key Research and Development Program of Shaanxi","award":["S2023-YF-YBSF-1579"],"award-info":[{"award-number":["S2023-YF-YBSF-1579"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Accurate detection of malignant cells in ovarian cancer ascites is essential for diagnosis and surgical staging. However, conventional cytological examination is hampered by low sensitivity and interpretive subjectivity. We evaluated a dual-modality strategy combining a near-infrared fluorescent zwitterionic folic acid (ZW-FA) probe targeting folate receptor \u03b1 with serum biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ascites and peritoneal lavage specimens were processed into cell blocks. A ZW-FA fluorescence cutoff was exploratorily derived using receiver operating characteristic curve analysis. Diagnostic performance of ZW-FA staining (quantified using ImageJ), hematoxylin-eosin (H&amp;E) staining, serum cancer antigen 125 (CA125), human epididymis protein 4, and the risk of ovarian malignancy algorithm was assessed individually and in combination. Postoperative histopathological diagnosis served as the reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among 80 participants, the optimal ZW-FA cutoff was 48.40 (area under the curve = 0.881). Automated fluorescence quantification achieved 100.00% technical reproducibility, providing an objective and consistent readout. ZW-FA correctly classified 2 of 5 (40.00%) specimens that were non-diagnostic by H&amp;E because of insufficient cellularity. In the complete-case comparative analysis of 65 participants, ZW-FA showed higher diagnostic accuracy than H&amp;E staining (86.15% vs. 81.54%). The combination of ZW-FA staining and serum CA125 achieved a sensitivity of 100.00%, a specificity of 81.25%, and an accuracy of 95.38%, exceeding the accuracy of all H&amp;E-based dual-modality strategies (\u2264 90.77%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The ZW-FA\/CA125 dual-modality strategy may improve the diagnostic assessment of ovarian cancer ascites and peritoneal lavage specimens, particularly in paucicellular samples. Further validation in larger, independent multicenter cohorts is warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15330338261488986","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:11:03Z","timestamp":1789679463000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Near-Infrared Fluorescent Zwitterionic Folic Acid Probe and Serum CA125 for Enhancing Diagnostic Accuracy of Malignant Ovarian Cancer Ascites"],"prefix":"10.1177","volume":"25","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxin","family":"Liang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanbo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Peking University People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadi","family":"Bin","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruining","family":"Ma","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3763-4456","authenticated-orcid":false,"given":"Qiling","family":"Li","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21820"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-020-05553-y"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2023.189052"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-0875-x"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz062"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2022.0047"},{"key":"e_1_3_8_8_2","doi-asserted-by":"publisher","DOI":"10.4240\/wjgs.v4.i4.87"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0339(199902)20:2<57::aid-dc2>3.0.co;2-m"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2004.01.035"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1159\/000528191"},{"issue":"1","key":"e_1_3_8_12_2","first-page":"43","article-title":"Detection of carcinoma in serous effusions: a review","volume":"11","author":"Li M","year":"2021","unstructured":"Li M, Zhao L, Zhou X, et al. 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