{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:08:59Z","timestamp":1789002539645,"version":"build-2803163510"},"reference-count":16,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T00:00:00Z","timestamp":1466640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Technol Cancer Res Treat"],"published-print":{"date-parts":[[2016,12]]},"abstract":"\n Objective:<\/jats:title>\n \n This objective of this study is to investigate the effects of the radiolabeled cyclic peptide\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC) on MHCC97-H human liver cancer cells in vitro and in vivo.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n The protein expression levels were examined by Western blot analysis. Biological activity of\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC) was assessed with the radioligand binding assay and competitive inhibition experiment. Subcellular localization of the cyclic peptide was observed by fluorescence microscopy. Animals were implanted with MHCC97-H cells and administered with\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC). Hematoxylin and eosin staining, electron microscopy, and immunohistochemistry were performed to evaluate the effects of\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC) on implanted tumors.\n <\/jats:p>\n <\/jats:sec>\n \n Result:<\/jats:title>\n \n The expression levels of interleukin 11 receptor were significantly elevated, by 2-to 5-fold, in tumor cell lines, especially for MHCC97-H cells. Characterization of\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC) showed that the biological activity of the cyclic peptide was not altered after labeling, and the radiolabeled cyclic peptide exhibited sufficient binding affinity to interleukin 11 receptor . The cyclic peptide of c(CGRRAGGSC) was mainly distributed in the cytoplasm and on the cell membrane of MHCC97-H cells. The in vivo experiments showed that the tumor growth was significantly inhibited by the treatment of\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC). The inhibitory effect of\n 153<\/jats:sup>\n Sm-DTPA-c(CGRRAGGSC) on tumor growth was further confirmed by Hematoxylin and eosin staining, electron microscopy, and immunohistochemistry. Moreover, the expression levels of interleukin 11 receptor in implanted tumors were significantly decreased in the treatment groups.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n 153<\/jats:sup>\n Sm-DTPA-c (CGRRAGGSC) could specifically bind to interleukin 11 receptor on MHCC97-H liver tumor cells, inhibiting the cell proliferation and inducing cellular apoptosis. These findings provide experimental evidence for the development of individual treatment of liver cancers, as well as recurrence and metastasis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/1533034615604785","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T20:38:32Z","timestamp":1442435912000},"page":"NP1-NP9","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Binding Specificity of Radiolabeled Cyclic Peptide\n 153<\/sup>\n Sm-DTPA-c(CGRRAGGSC) to MHCC97-H Human Liver Cancer Cells and its Antitumor Effects\n in vivo<\/i>"],"prefix":"10.1177","volume":"15","author":[{"given":"Qinghua","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Radiology, the Third Affiliated Hospital of Nantong University, Wuxi, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"He","sequence":"additional","affiliation":[{"name":"Department of Radiology, the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Radiology, the Third Affiliated Hospital of Nantong University, Wuxi, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Radiology, the Third Affiliated Hospital of Nantong University, Wuxi, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Interventional Radiology, the Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, 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