{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:33:04Z","timestamp":1791592384068,"version":"4.3.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2022,4,24]],"date-time":"2022-04-24T00:00:00Z","timestamp":1650758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["00023001"],"award-info":[{"award-number":["00023001"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant Agency of Charles University","award":["GAUK 304321"],"award-info":[{"award-number":["GAUK 304321"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The International Journal of Lower Extremity Wounds"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:p>\n                    Autologous cell therapy (ACT) is a new therapeutic approach for diabetic patients with no-option chronic limb-threatening ischemia (NO-CLTI). The aim of our study was to quantify cell populations of cell therapy products (CTPs) obtained by three different isolation methods and to correlate their numbers with changes in transcutaneous oxygen pressure (TcPO\n                    <jats:sub>2<\/jats:sub>\n                    ). CTPs were separated either from stimulated peripheral blood (PB) (n\u2009=\u200911) or harvested from bone marrow (BM) processed either by Harvest SmartPReP2 (n\u2009=\u200950) or sedimented with succinate gelatin (n\u2009=\u200929). The clinical effect was evaluated by the change in TcPO\n                    <jats:sub>2<\/jats:sub>\n                    after 1, 3 and 6 months. TcPO\n                    <jats:sub>2<\/jats:sub>\n                    increased significantly in all three methods at each time point in comparison with baseline values (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.01) with no significant difference among them. There was no correlation between the change in TcPO\n                    <jats:sub>2<\/jats:sub>\n                    and the size of injected cell populations. We only observed a weak correlation between the number of injected white blood cells (WBC) and an increase in TcPO\n                    <jats:sub>2<\/jats:sub>\n                    at 1 and 3 months. Our study showed that all three isolation methods of ACT were similarly relatively efficient in the treatment of NO-CLTI. We observed no correlation of TcPO\n                    <jats:sub>2<\/jats:sub>\n                    increase with the number of injected monocytes, lymphocytes or CD34\n                    <jats:sup>+<\/jats:sup>\n                    . We observed a weak correlation between TcPO\n                    <jats:sub>2<\/jats:sub>\n                    increase and the number of injected WBCs.\n                  <\/jats:p>","DOI":"10.1177\/15347346221095954","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T02:07:37Z","timestamp":1650852457000},"page":"383-391","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of Three Methods for Preparation of Autologous Cells for Use in Cell Therapy of Chronic Limb-Threatening Ischemia in People with Diabetes"],"prefix":"10.1177","volume":"24","author":[{"given":"Jitka","family":"Husakova","sequence":"first","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"},{"name":"First Faculty of Medicine, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Bem","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Jirkovska","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Nemcova","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimira","family":"Fejfarova","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karol","family":"Sutoris","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3854-0390","authenticated-orcid":false,"given":"Michal","family":"Kahle","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Jude","sequence":"additional","affiliation":[{"name":"Diabetes Center, Tameside Hospital NHS Foundation Trust and University of Manchester, Lancashire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2078-0177","authenticated-orcid":false,"given":"Michal","family":"Dubsky","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Medicine, Prague, Czech Republic"},{"name":"First Faculty of Medicine, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,4,24]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/2000625X.2019.1696012"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/1708538118786864"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-014-0140-x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0938-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foot.2019.04.003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181fc3c73"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2019.05.006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2014.08.010"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2010.08.060"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719835177"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2020.02.007"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.08.038"},{"key":"e_1_3_2_14_2","article-title":"Autologous cells derived from different sources and administered using different regimens for \u201cno-option\u201d critical lower limb ischaemia patients","volume":"8","author":"Wahid SF A","year":"2018","unstructured":"Wahid SF A, Ismail NA, Wan Jamaludin WF, et al. 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