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The formation of biofilms on wounds significantly contributes to infections and delayed healing. While existing methods for identifying these biofilms have limitations, there is a need for a convenient tool for its clinical application. This literature review aimed to address the problem with current clinical biofilm identification in wound care and a proposal for biofilm-detection-based wound care in diabetic foot ulcer patients. Identifying biofilms is particularly vital due to the absence of typical signs of infection in DFUs. However, current approaches, although effective, often prove invasive and technically intricate. The wound blotting technique, involving attaching a nitrocellulose membrane and subsequent staining, presents an alternative that is swift and non-invasive. Research highlights the applicability of wound blotting with alcian blue staining in clinical scenarios, consistently producing sensitive outcomes. By addressing the critical need for early biofilm detection, wound blotting holds promise for enhancing DFU management and contributing to strategies aimed at preventing amputations.<\/jats:p>","DOI":"10.1177\/15347346241273112","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T04:21:57Z","timestamp":1723177317000},"page":"401-406","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Challenges in Biofilm Identification in Diabetic Foot Infections: Review of Literature"],"prefix":"10.1177","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3582-7556","authenticated-orcid":false,"given":"Adam","family":"Astrada","sequence":"first","affiliation":[{"name":"Esa Unggul University"},{"name":"The University of Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gojiro","family":"Nakagami","sequence":"additional","affiliation":[{"name":"The University of Tokyo"},{"name":"The University of Tokyo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Sanada","sequence":"additional","affiliation":[{"name":"The University of Tokyo"},{"name":"The University of Tokyo"},{"name":"Ishikawa Prefectural Nursing University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"e_1_3_4_2_2","volume-title":"IDF Diabetes Atlas","author":"International Diabetes Federation","year":"2019","unstructured":"International Diabetes Federation. 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