{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:16:26Z","timestamp":1777695386481,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Jiangxi Provincial Administration of Traditional Chinese Medicine","award":["2022Z026"],"award-info":[{"award-number":["2022Z026"]}]},{"DOI":"10.13039\/501100003453","name":"natural science foundation of guangdong province","doi-asserted-by":"publisher","award":["2022A1515011260"],"award-info":[{"award-number":["2022A1515011260"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012245","name":"science and technology planning project of guangdong province","doi-asserted-by":"publisher","award":["2020B1111120001"],"award-info":[{"award-number":["2020B1111120001"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010256","name":"guangzhou municipal science and technology project","doi-asserted-by":"publisher","award":["202102080232"],"award-info":[{"award-number":["202102080232"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ganzhou Science and Technology Plan Project","award":["2023LNS27059"],"award-info":[{"award-number":["2023LNS27059"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Integr Cancer Ther"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Objective: The Chinese medicine Jianpi-Huayu decoction (\u5065\u813e\u5316\u7600\u65b9, JPHY) can alleviate cancer-related fatigue in patients with liver cancer. However, its mechanism remains unclear. In this study, we used BALB\/c mice with liver cancer model to investigate whether JPHY alleviates cancer-related fatigue by regulating Th1\/Th2 immune balance; and the possible association with the IL-27\/STAT1 signaling pathway. Methods: We established a mouse model of liver cancer fatigue. Mice were gavaged with physiological saline, low, medium, or high concentrations of JPHY respectively; and intraperitoneal injection of fludarabine (STAT1 pathway inhibitor) with JPHY for 21\u2009days. We recorded the general condition of the mice, and assessed fatigue using scoring criteria and Exhausted Swimming Test. We calculated the spleen and thymus indices, performed H&amp;E staining and immunohistochemical analysis on liver tumor tissues to observe the tumor proliferation marker ki67. We quantified the secretion levels of IFN-\u03b3 and IL-2 produced by Th1 cells in serum and splenic lymphocytes, as well as the secretion of IL-4, IL-10 by Th2 cells, and IL-27 in the signaling pathway through ELISA analysis. We evaluated the expression levels of p-STAT1 and STAT1 in spleen tissues using Western blot analysis. Results: JPHY exhibits a therapeutic effect on hepatocellular carcinoma-induced splenomegaly in murine models by upregulating the pro-inflammatory cytokines IFN-\u03b3 and IL-2 and downregulating the anti-inflammatory cytokines IL-4 and IL-10. Moreover, JPHY suppresses ki67 expression, reduces tumor-related inflammation infiltration, and ameliorates cancer-associated fatigue. Additionally, the expression of phosphorylated protein p-STAT1 is down-regulated. Conclusion: JPHY may improve the Th1\/Th2 immune balance through its anti-inflammatory effects and promotion of IL-27-induced STAT1 phosphorylation, thereby alleviating fatigue in mice with liver cancer.<\/jats:p>","DOI":"10.1177\/15347354241263018","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T05:07:46Z","timestamp":1722316066000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Jianpi Huayu Decoction on Th1\/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27\/STAT1 Signaling Pathway"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0751-9379","authenticated-orcid":false,"given":"Chen","family":"Jiayi","sequence":"first","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Siru","sequence":"additional","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luo","family":"Xiaoqi","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"},{"name":"School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Enling","sequence":"additional","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"},{"name":"School of Medicine, South China University of Technology, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Juze","sequence":"additional","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0420-4679","authenticated-orcid":false,"given":"Wang","family":"Changjun","sequence":"additional","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Traditional Chinese Medicine, Guangdong Geriatric Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,7,30]]},"reference":[{"key":"bibr1-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-023-00849-y"},{"key":"bibr2-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25294"},{"key":"bibr3-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21671"},{"key":"bibr4-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1002\/pon.1120"},{"key":"bibr5-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-020-0707-5"},{"key":"bibr6-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijnurstu.2020.103707"},{"key":"bibr7-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2012.06.011"},{"key":"bibr8-15347354241263018","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2012.04.004"},{"key":"bibr9-15347354241263018","first-page":"2597","volume":"9","author":"Yao 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