{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:20:42Z","timestamp":1777695642853,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"publisher","award":["81972695"],"award-info":[{"award-number":["81972695"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Introduction and Education Program for young Talents in Shandong Colleges and universities"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Integr Cancer Ther"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>This study delves into the therapeutic effects of combining GEM and Angelica polysaccharide (APS) on triple-negative breast cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In vitro, proliferation, apoptosis of 4T-1 cells and MDSC were detected by flow cytometry. Migration of 4T-1 cell was detected by scratch healing experiment after treatment by GEM (0, 2.5, 5 \u03bcM), APS (160,320 mg\/ml), or GEM + APS (2.5 \u03bcM + 160 mg\/ml, 5 \u03bcM + 320 mg\/ml). In vivo, 4T-1 cells were injected into the mammary fat pad under the mammary gland of BALB\/c mice to establish an orthotopic breast cancer tumor model. They were randomly divided into control group (0.9% normal saline + ultrapure water), GEM group (0.9% normal saline preparation, 100 mg\/kg, intraperitoneal injection twice a week), APS group (ultrapure water preparation, 200 mg\/kg, intraperitoneal injection once a day), GEM + APS group (GEM 100 mg\/kg, intraperitoneal injection twice a week and APS 200 mg\/kg, intraperitoneal injection once a day) for 3 weeks. The proportion of immune cells in the spleen and tumor microenvironment were detected by flow cytometry, immunofluorescence and Mindray hematology analyzer. The tumor volume and weight, spleen index were recorded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The in vitro experimental results revealed that GEM effectively inhibited the proliferation and migration of 4T-1 cells and induced apoptosis in both 4T-1 cells and MDSCs. In contrast, APS had no impact on 4T-1 cells or MDSCs. The in vivo experimental findings indicated that compared with the single-drug treatment groups, the combination treatment of GEM + APS more effectively regulated the proportion of peripheral and local anti-tumor MDSCs and T cells, and more significantly curbed the progression of breast cancer in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>APS can exert a synergistic effect through immune regulation to enhance the therapeutic efficacy of GEM on triple-negative breast cancer. It aims to offer novel insights for the clinical application of combining GEM with immunotherapy for patients with triple negative breast cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15347354251414897","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:16:06Z","timestamp":1771305366000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Effect of Gemcitabine Combined With Angelica Polysaccharide on Triple Negative Breast Cancer in Mice"],"prefix":"10.1177","volume":"25","author":[{"given":"Fan","family":"Lei","sequence":"first","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Inflammatory Disease Research in Universities of Shandong Province, Shandong Second Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiling","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Inflammatory Disease Research in Universities of Shandong Province, Shandong Second Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yating","family":"Shu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Inflammatory Disease Research in Universities of Shandong Province, Shandong Second Medical University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Weifang People\u2019s Hospital, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, Yantai Yuhuangding Hospital, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhentao","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Inflammatory Disease Research in Universities of Shandong Province, Shandong Second Medical University, China"},{"name":"Department of Nephrology, Affiliated Hospital of Shandong Second Medical University, Weifang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3768-5379","authenticated-orcid":false,"given":"Meiyu","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Inflammatory Disease Research in Universities of Shandong Province, Shandong Second Medical University, China"},{"name":"Department of Nephrology, Affiliated Hospital of Shandong Second Medical University, Weifang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12207"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01341-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.115.157834"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.20751"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2022.2151584"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.5644\/ama2006-124.328"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2147\/bctt.S69488"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-025-02305-x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes14051008"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.06.002"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020779"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a036863"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01805-y"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25147517"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.05.023"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.5483\/bmbrep.2007.40.5.636"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.01.004"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.09.213"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.12.050"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202100089"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2006.02.013"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.989230"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-15-0224"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-024-01990-4"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes13030466"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1536428"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2009.03.015"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3724\/abbs.2023040"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155656"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113965"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.3791\/54040"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-16-0297"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.11.015"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05134-6"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1368687"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-023-00846-y"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-016-0167-7"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/COC.0b013e3182a530dd"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1177\/09603271211002883"}],"container-title":["Integrative Cancer Therapies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15347354251414897","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15347354251414897","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15347354251414897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:18:04Z","timestamp":1777454284000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15347354251414897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":39,"alternative-id":["10.1177\/15347354251414897"],"URL":"https:\/\/doi.org\/10.1177\/15347354251414897","relation":{},"ISSN":["1534-7354","1552-695X"],"issn-type":[{"value":"1534-7354","type":"print"},{"value":"1552-695X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"article-number":"15347354251414897"}}