{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:29:40Z","timestamp":1787862580214,"version":"build-2784847793"},"reference-count":46,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>\n                    Foodborne botulism is a rare but highly lethal disease. The\n                    <jats:italic toggle=\"yes\">botulinum<\/jats:italic>\n                    neurotoxin (BoNT) that causes botulism is produced by\n                    <jats:italic toggle=\"yes\">Clostridium botulinum (C. botulinum)<\/jats:italic>\n                    and other\n                    <jats:italic toggle=\"yes\">clostridia<\/jats:italic>\n                    . In this study, we characterized\n                    <jats:italic toggle=\"yes\">C. botulinum<\/jats:italic>\n                    strains isolated during food poisoning events in Sichuan Province from 1990 to 2024 by analyzing whole-genome sequencing data. Statistical analyses of the geographical distribution food sources of strains suggested that the primary sources of contamination were associated with specific regions and food types. The isolates were further compared with reference strains using average nucleotide identity analysis to reveal their genetic diversity and evolutionary relationships. Among the eight strains, five strains belonged to Group I, and the other three strains belonged to Group II. All strains belonging to Group I (SC001, SC002, SC003, SC006, and SC009) were assigned to five totally different recognized ST types (ST-2 to ST-51). In addition, analysis of BoNTs subtypes demonstrated that the types of BoNTs causing botulism in Sichuan were mainly types A, B, and E. Among them, some rare subtypes of BoNT reported for the first time in China, such as BoNT\/B4, BoNT\/E12, and BoNT\/A5(B3), and no specific subtypes were predominant in the botulism incidents. This study is critical for disease surveillance and early warning systems, while also providing a basis for food safety regulation, clinical diagnosis, and treatment in the future.\n                  <\/jats:p>","DOI":"10.1177\/15353141251365831","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T18:00:26Z","timestamp":1759255226000},"page":"20-28","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Characterization of\n                    <i>Clostridium botulinum<\/i>\n                    Isolates from Sichuan Province, China (1990\u20132024)"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9542-9760","authenticated-orcid":false,"given":"Shiyan","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaopeng","family":"Lei","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention of Sichuan Province, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.8.1059"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.278830.123"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00072-11"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00469-08"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12050338"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.09.001"},{"key":"e_1_3_3_8_1","unstructured":"Control ECfDPa. Botulism-Annual Epidemiological Report for 2022. 2024. Available from: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/botulism-annual-epidemiological-report-2022 [Last accessed: July 10]."},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00468-10"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02422-10"},{"issue":"3","key":"e_1_3_3_11_1","article-title":"A review of botulism in China","volume":"3","author":"Gao QY","year":"1990","unstructured":"Gao QY, , Huang YF, , Wu JG, et al. A review of botulism in China. Biomed Environ Sci, 1990; 3(3).","journal-title":"Biomed Environ Sci"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins15020092"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2018.1524582"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2024.06.018"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2001.0468"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2015.09.011"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-014-0887-4"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12969"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/hs.2023.0081"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.46234\/ccdcw2022.114"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12080509"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.645248"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1567360"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.02942-14"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9010038"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12081580"},{"key":"e_1_3_3_28_1","unstructured":"Prevention USCfDCa. 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