{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:54:53Z","timestamp":1792065293332,"version":"4.4.0"},"reference-count":23,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"National Fund project","award":["No.32160852"],"award-info":[{"award-number":["No.32160852"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2026,12,1]]},"abstract":"<jats:p>\n                    Quinolones are one of the most widely used antibiotics globally for the treatment of\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    infections, and resistance to their various generations of drugs occurs. The present study aimed to investigate the inhibitory effect of quercetin on quinolone-resistant\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    , triggered by efflux pumps, to assess its potential value as an efflux pump inhibitor. The minimum inhibitory concentration of ciprofloxacin for resistant bacteria was determined by the micro broth dilution method, combined with UV spectrophotometry and high-performance liquid chromatography, to observe the changes in ciprofloxacin accumulation in the antibiotic-resistant bacteria after treatment with quercetin, quantitative real-time PCR (qRT-PCR) was used to analyze the transcript levels of some efflux pump genes, finally, the whole proteins of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    were extracted by the action of quercetin, following bicinchoninic acid (BCA) quantification, proteins were separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and overall changes in total protein bands were observed. The results showed that 64\u2013256 \u00b5g\/mL quercetin significantly increased the accumulation of ciprofloxacin in antibiotic-resistant bacteria, inhibited the efflux of ciprofloxacin by 2\u201310-fold downregulation of the expression of the efflux pump genes\n                    <jats:italic toggle=\"yes\">norA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">norB<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">norC<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">mepA<\/jats:italic>\n                    , and modulated the protein expression profile of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    . This study revealed that quercetin has the potential to act as an efflux pump inhibitor, providing a theoretical basis for the treatment of antibiotic-resistant bacterial infections.\n                  <\/jats:p>","DOI":"10.1177\/15353141251394587","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T09:36:36Z","timestamp":1762767396000},"page":"869-876","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of the Effect of Quercetin on the Accumulation of Ciprofloxacin Resistance in\n                    <i>Staphylococcus aureus<\/i>"],"prefix":"10.1177","volume":"23","author":[{"given":"Haorong","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panpan","family":"Liu","sequence":"additional","affiliation":[{"name":"Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingge","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junpeng","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13100677"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-00994-9"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7977433"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14404"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2019.103291"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-021-09886-4"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2008.00063.x"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2010.02267.x"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12830"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0287973"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14050424"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00187-22"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1074559"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413363"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-024-03980-7"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b00077"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13015"},{"issue":"2","key":"e_1_3_3_19_1","first-page":"148","article-title":"An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"8","author":"Puissant C","year":"1990","unstructured":"Puissant C, , Houdebine LM. An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Biotechniques, 1990; 8(2):148\u2013149.","journal-title":"Biotechniques"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00007-19"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.7.2395-2405.2005"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.7238"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/MSF.980.197"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-025-03856-1"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353141251394587","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15353141251394587","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353141251394587","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:18:29Z","timestamp":1792063109000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15353141251394587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,10]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,12,1]]}},"alternative-id":["10.1177\/15353141251394587"],"URL":"https:\/\/doi.org\/10.1177\/15353141251394587","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,10]]}}}