{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:36:49Z","timestamp":1777642609341,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                    is an important bacterial pathogen transmitted through milk and dairy products. This study that aimed to detect\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    contamination of raw milk using polymerase chain reaction (PCR) is the first PCR-based detection of\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    from raw milk in Ogun and Oyo States. Twenty-six bovine milk samples were collected for analysis.\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolation, molecular identification, antimicrobial susceptibility testing, and plasmid detection were done using standard bacteriological, Kirby\u2013Bauer disc diffusion, and molecular methods, respectively. Eighteen samples were presumptively positive for\n                    <jats:italic toggle=\"yes\">Listeria<\/jats:italic>\n                    species. Of the 18 isolates, molecular analysis of the\n                    <jats:italic toggle=\"yes\">actA<\/jats:italic>\n                    gene confirmed 5\/18 (27.8%) as\n                    <jats:italic toggle=\"yes\">L. monocytogenes.<\/jats:italic>\n                    All\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolates were resistant to erythromycin, cefuroxime, and amoxicillin. Four (80%) and 1 (20%) showed resistance to imipenem and cefepime, respectively. Although all\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolates were susceptible to ciprofloxacin, all isolates were multidrug resistant. Only 1\/5 (20%) isolates possessed plasmid (size, 12.2 kb). While the high milk contamination highlights a potential\n                    <jats:italic toggle=\"yes\">Listeria<\/jats:italic>\n                    outbreak, the high rate of multidrug resistance and plasmid possession observed among\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolates underscore a serious public health threat due the high chances of disseminating plasmids that mediate antimicrobial resistance to other bacteria and the difficulty in treatment if implicated in infection. However, PCR detection efficiency indicates its effectiveness in enhancing surveillance that could reduce public health impact of listeriosis.\n                  <\/jats:p>","DOI":"10.1177\/15353141261438103","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T10:11:36Z","timestamp":1774692696000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of\n                    <i>Listeria monocytogenes<\/i>\n                    from Raw Milk (Nono) in Cows Using Polymerase Chain Reaction"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9569-088X","authenticated-orcid":false,"given":"Joan L.","family":"Ojomo","sequence":"first","affiliation":[{"name":"Mountain Top University, Department of Biological Sciences, Ibafo, Ogun State, Nigeria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel K.","family":"Olukoya","sequence":"additional","affiliation":[{"name":"Mountain Top University, Department of Biological Sciences, Ibafo, Ogun State, Nigeria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Utibeima U.","family":"Essiet","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research, Yaba, Lagos."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Ajayi","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research, Yaba, Lagos."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stella I.","family":"Smith","sequence":"additional","affiliation":[{"name":"Mountain Top University, Department of Biological Sciences, Ibafo, Ogun State, Nigeria."},{"name":"Nigerian Institute of Medical Research, Yaba, Lagos."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,28]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1653368"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2019.1691364"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001445"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-023-01427-6"},{"key":"e_1_3_3_6_1","unstructured":"Center for Disease Commission. Listeria infection (listeriosis). 2024. Available from: https:\/\/www.cdc.gov\/listeria\/about\/index.html [Last accessed: May2 2024]."},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S526926"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.13234"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13102280"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.11648\/j.avs.20140206.12"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.5923\/J.MICROBIOLOGY.20160601.04"},{"issue":"1","key":"e_1_3_3_12_1","first-page":"82","article-title":"Prevalence and molecular detection of Listeria Monocytogenes from raw cow milk and poultry droppings in Jos, Nigeria","volume":"3","author":"Goyil G","year":"2022","unstructured":"Goyil G, , Nkup A, , Yunzoom J, et al. Prevalence and molecular detection of Listeria Monocytogenes from raw cow milk and poultry droppings in Jos, Nigeria. Hmlyn Jr Appl Med Scie Res 2022;3(1):82\u201387.","journal-title":"Hmlyn Jr Appl Med Scie Res"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07845-6"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/5590136"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-022-03214-7"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-39955-0"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005437"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2020.107540"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2019-038"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2025.104745"},{"issue":"2","key":"e_1_3_3_21_1","article-title":"Bacteriological quality of Nono, a milk product sold at retail outlets in Federal Capital Territory, Nigeria","volume":"3","author":"Uzoaga GO","year":"2020","unstructured":"Uzoaga GO, , Umeokonkwo CD, , Usman AB, et al. Bacteriological quality of Nono, a milk product sold at retail outlets in Federal Capital Territory, Nigeria. J Interval Epidemiol Public Health 2020;3(2); doi: 10.37432\/JIEPH.2020.3.2.251","journal-title":"J Interval Epidemiol Public Health"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11756-024-01697-3"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.4314\/sokjvs.v10i2.3"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353141261438103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15353141261438103","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353141261438103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:23:38Z","timestamp":1777443818000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15353141261438103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,28]]},"references-count":22,"alternative-id":["10.1177\/15353141261438103"],"URL":"https:\/\/doi.org\/10.1177\/15353141261438103","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,28]]},"article-number":"15353141261438103"}}