{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T18:33:52Z","timestamp":1792953232214,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Natural Science Foundation of Guizhou Province","award":["ZK[2022]309"],"award-info":[{"award-number":["ZK[2022]309"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic toggle=\"yes\">Streptococcus agalactiae<\/jats:italic>\n                    (Group B\n                    <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                    ) is a leading causative agent of sepsis, pneumonia and meningitis in neonates and is also a major pathogen responsible for bovine mastitis, posing a substantial threat to food safety and public health via the dairy production chain. The ability of\n                    <jats:italic toggle=\"yes\">S. agalactiae<\/jats:italic>\n                    to form biofilms in the host enhances its resistance to conventional antibiotics and host immune defenses, thereby contributing to persistent and chronic infections. This study aimed to evaluate the antibacterial, antibiofilm, and combined antibacterial effects of dihydromyricetin (DMY) against the\n                    <jats:italic toggle=\"yes\">S. agalactiae<\/jats:italic>\n                    strain GBS2 isolated from bovine mastitis. DMY exhibited significant antibacterial activity against GBS2, with a minimum inhibitory concentration and minimum bactericidal concentration of 0.5 mg\/mL. Crystal violet staining and confocal laser scanning microscopy analyses demonstrated that DMY effectively inhibited and disrupted biofilm formation, resulting in looser and less dense biofilm structures compared to untreated controls. Furthermore, reverse transcription-quantitative PCR (RT-qPCR) results revealed that DMY modulated the transcript levels of biofilm-associated genes (\n                    <jats:italic toggle=\"yes\">csrR<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">srtA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">fbsC<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">cpsE<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">luxS<\/jats:italic>\n                    ). Additionally, DMY markedly potentiated the antibacterial effect of penicillin G, ciprofloxacin, oxacillin, and vancomycin. These findings suggest that DMY could serve as a natural antibiofilm agent for controlling\n                    <jats:italic toggle=\"yes\">S. agalactiae<\/jats:italic>\n                    contamination in the dairy industry, thereby contributing to the prevention of foodborne infections and reducing reliance on conventional antibiotics.\n                  <\/jats:p>","DOI":"10.1177\/15353141261501298","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T08:42:06Z","timestamp":1792658526000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Dihydromyricetin as an Antibiofilm and Antibacterial Agent Against\n                    <i>Streptococcus agalactiae<\/i>"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7506-8927","authenticated-orcid":false,"given":"Ronghua","family":"Ma","sequence":"first","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leitao","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixiong","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guizhou Normal University, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingbei","family":"Weng","sequence":"additional","affiliation":[{"name":"Qiannan Normal University for Nationalities, Duyun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-00114-z"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158809"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007421"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.553073"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.02.016"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-9899"},{"key":"e_1_3_3_8_1","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically","author":"CLSI","year":"2018","unstructured":"CLSI. 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