{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T19:54:09Z","timestamp":1788378849353,"version":"build-2803163510"},"reference-count":37,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017618","name":"Division of Diabetes, Endocrinology, and Metabolic Diseases","doi-asserted-by":"publisher","award":["K01DK118005"],"award-info":[{"award-number":["K01DK118005"]}],"id":[{"id":"10.13039\/100017618","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Imaging"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In rodents, 11\u03b2-hydroxysteroid dehydrogenase 1 (11\u03b2-HSD1) catalyzes the conversion of inactive 11-dehydrocorticosterone to the active hormone corticosterone. Dysregulation of intracellular glucocorticoid action is implicated in metabolic diseases. Assessing 11\u03b2-HSD1 enzyme levels in vivo may be key to understanding obesity pathophysiology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      We used a Zucker Fatty (ZF) rat model and [\n                      <jats:sup>18<\/jats:sup>\n                      F]AS2471907 PET imaging to determine appropriate kinetic modeling methods and assess changes in 11\u03b2-HSD1 levels due to obesity in the liver, white and brown adipose tissue (WAT\/BAT), and brain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      To validate [\n                      <jats:sup>18<\/jats:sup>\n                      F]AS2471907 PET in preclinical models, time-activity curves (TACs) were generated and kinetic modeling was performed with image-derived input functions (IDIFs) extracted from multiple locations. Quantitative estimates of radioligand binding were compared with ex vivo 11\u03b2-HSD1 protein expression. Validated quantitative PET kinetic modeling methods were then used to assess differences in 11\u03b2-HSD1 between lean and obese ZF rats. Metabolic disease status was confirmed with stable isotopes tracer studies of glucose and fatty acid metabolism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Obesity is associated with decreased brain 11\u03b2-HSD1 levels, measured by [\n                      <jats:sup>18<\/jats:sup>\n                      F]AS2471907 PET, which correlated with measures of glucose and fatty acid metabolism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      We demonstrate that [\n                      <jats:sup>18<\/jats:sup>\n                      F]AS2471907 PET can provide useful quantification of 11\u03b2-HSD1 levels in a rodent model of obesity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15353508241301584","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T09:15:46Z","timestamp":1734513346000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantification of Multi-Organ  11\u03b2-Hydroxysteroid Dehydrogenase  Type 1 Enzyme Levels in a Zucker  Fatty Rat Model: A PET Imaging Study"],"prefix":"10.1177","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4047-6575","authenticated-orcid":false,"given":"Jason","family":"Bini","sequence":"first","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Strober","sequence":"additional","affiliation":[{"name":"Section of Endocrinology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kapinos","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Qiang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songye","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Ropchan","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabeel","family":"Nabulsi","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel J.","family":"Perry","sequence":"additional","affiliation":[{"name":"Section of Endocrinology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA"},{"name":"Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2073-0273","authenticated-orcid":false,"given":"Daniel F.","family":"Vatner","sequence":"additional","affiliation":[{"name":"Section of Endocrinology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard E.","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Radiology and Biomedical Imaging, Yale University School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"bibr1-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00020.2012"},{"key":"bibr2-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2021.111210"},{"key":"bibr3-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-1412"},{"key":"bibr4-15353508241301584","doi-asserted-by":"publisher","DOI":"10.2337\/db08-1404"},{"key":"bibr5-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.2.7297"},{"key":"bibr6-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2018.12.003"},{"key":"bibr7-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.071562698"},{"issue":"3","key":"bibr8-15353508241301584","first-page":"1022","volume":"84","author":"Stewart PM","year":"1999","journal-title":"J Clin Endocrinol Metab"},{"key":"bibr9-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.3.7453"},{"issue":"7","key":"bibr10-15353508241301584","first-page":"3330","volume":"87","author":"Rask E","year":"2002","journal-title":"J Clin Endocrinol Metab"},{"key":"bibr11-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030596"},{"key":"bibr12-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.09.010"},{"key":"bibr13-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.12.013"},{"key":"bibr14-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-23-0034"},{"issue":"1","key":"bibr15-15353508241301584","first-page":"277","volume":"87","author":"Andrew R","year":"2002","journal-title":"J Clin Endocrinol Metab"},{"key":"bibr16-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-014-3240-x"},{"key":"bibr17-15353508241301584","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.5.1364"},{"key":"bibr18-15353508241301584","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0969"},{"key":"bibr19-15353508241301584","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.118.219766"},{"key":"bibr20-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.8b00715"},{"key":"bibr21-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19838633"},{"key":"bibr22-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1172\/JCI150452"},{"key":"bibr23-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-020-01490-z"},{"key":"bibr24-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1109\/TRPMS.2021.3088606"},{"key":"bibr25-15353508241301584","volume-title":"Liver and brain levels of 11\u03b2-hydroxysteroid dehydrogenase type 1 enzyme in obesity: Preliminary results from PET imaging studies","author":"Bini J"},{"key":"bibr26-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(00)00125-6"},{"key":"bibr27-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600493"},{"key":"bibr28-15353508241301584","unstructured":"Carson RE. T\n                      racer kinetic modeling in PET: Basic science and clinical practice\n                      . 3rd ed. Positrion Emission Tomography; 2003:147\u2013179."},{"key":"bibr29-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000038000.34930.4E"},{"key":"bibr30-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1990.127"},{"key":"bibr31-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.12.001"},{"key":"bibr32-15353508241301584","doi-asserted-by":"publisher","DOI":"10.3390\/nu12051356"},{"key":"bibr33-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1002\/hbm.20870"},{"key":"bibr34-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704144200"},{"key":"bibr35-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-0117"},{"key":"bibr36-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2017.80"},{"key":"bibr37-15353508241301584","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802706R"}],"container-title":["Molecular Imaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353508241301584","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15353508241301584","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15353508241301584","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:27:13Z","timestamp":1777703233000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15353508241301584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":37,"alternative-id":["10.1177\/15353508241301584"],"URL":"https:\/\/doi.org\/10.1177\/15353508241301584","relation":{},"ISSN":["1535-3508","1536-0121"],"issn-type":[{"value":"1535-3508","type":"print"},{"value":"1536-0121","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"15353508241301584"}}