{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:34:43Z","timestamp":1777872883005,"version":"3.51.4"},"reference-count":25,"publisher":"Frontiers Media SA","issue":"11","license":[{"start":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T00:00:00Z","timestamp":1378252800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>\n                    The influence of the histone deacetylase (HDAC)-inhibitor, valproic acid (VPA), on bladder cancer cell adhesion in\u00a0vitro was investigated in this paper. TCCSUP and RT-112 bladder cancer cells were treated with VPA (0.5 or 1\u2009mM) twice or thrice weekly for 14 days. Controls remained untreated. Tumour cell interaction with immobilized collagen was evaluated by a flow-based adhesion assay using a shear force of 2 or 4\u2009dyne\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . The effects of VPA on the integrin adhesion receptors \u03b13, \u03b15, \u03b21, \u03b23 and \u03b24 were assessed by flow cytometry to determine integrin surface expression and by western blotting to determine the cytoplasmic integrin level. VPA of 0.5\u2009mM and 1\u2009mM significantly prevented binding of both RT-112 and TCCSUP cells to collagen, compared with the untreated controls. Adhesion was reduced to a higher extent when RT-112 (subjected to 2\u2009dyne\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) or TCCSUP (subjected to 2 or 4\u2009dyne\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) tumour cells were treated with VPA three times a week, compared to the two times a week protocol. VPA caused a significant up-regulation of the integrin \u03b13, \u03b15, \u03b21, \u03b23 and \u03b24 subtypes on the TCCSUP cell surface membrane. In RT-112 cells, only integrin \u03b15 was elevated on the cell surface following VPA exposure. Western blotting revealed an up-regulation of \u03b13, \u03b15, \u03b23 and \u03b24 integrins and down-regulation of the integrin \u03b21 protein by VPA in TCCSUP. VPA also up-regulated \u03b15 and down-regulated \u03b21 integrin in RT-112 cells, but also reduced \u03b13 and \u03b23 in TCCSUP. VPA exerted adhesion-blocking properties on bladder cancer cells under physiologic flow conditions. The effects were accompanied by distinct modifications of the integrin expression profile, which differ depending on the cell lines used. Application of VPA might be an innovative option to prevent bladder cancer dissemination.\n                  <\/jats:p>","DOI":"10.1177\/1535370213498975","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T23:07:17Z","timestamp":1378336037000},"page":"1297-1304","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["HDAC inhibition suppresses bladder cancer cell adhesion to collagen under flow conditions"],"prefix":"10.3389","volume":"238","author":[{"given":"Eva","family":"Juengel","sequence":"first","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha Meyer dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmina","family":"Makarevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukasz","family":"Hudak","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Bartsch","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel","family":"Haferkamp","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Wiesner","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman A","family":"Blaheta","sequence":"additional","affiliation":[{"name":"Department of Urology, Johann Wolfgang Goethe-University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2013,9,4]]},"reference":[{"key":"bibr1-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1007\/s00120-006-1065-z"},{"key":"bibr2-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08795.x"},{"key":"bibr3-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-4-17"},{"key":"bibr4-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2008.11.018"},{"key":"bibr5-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-5-49"},{"key":"bibr7-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0b013e32834a2c70"},{"key":"bibr8-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.8.2704.420k41_2704_2715"},{"key":"bibr9-1535370213498975","doi-asserted-by":"publisher","DOI":"10.3109\/09537100903410609"},{"key":"bibr10-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.04.001"},{"key":"bibr11-1535370213498975","first-page":"1016","volume":"144","author":"Liebert M","year":"1994","journal-title":"Am J Pathol"},{"key":"bibr12-1535370213498975","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2000_3968"},{"key":"bibr13-1535370213498975","first-page":"347","volume":"18","author":"Oertl A","year":"2006","journal-title":"Int J Mol Med"},{"key":"bibr14-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-011-9386-8"},{"key":"bibr15-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-005-4335-z"},{"key":"bibr16-1535370213498975","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2002_3773"},{"key":"bibr17-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1996.57"},{"key":"bibr18-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2006.01.046"},{"key":"bibr19-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.12.012"},{"key":"bibr20-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-5-4"},{"key":"bibr22-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201012126"},{"key":"bibr23-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200106070"},{"key":"bibr24-1535370213498975","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.090028"},{"key":"bibr25-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1155\/1997\/246136"},{"key":"bibr26-1535370213498975","first-page":"38","volume":"57","author":"Kim SY","year":"1997","journal-title":"Cancer Res"},{"key":"bibr27-1535370213498975","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)61685-9"}],"container-title":["Experimental Biology and Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1535370213498975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1535370213498975","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1535370213498975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T17:46:52Z","timestamp":1777571212000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ebm-journal.org\/journals\/experimental-biology-and-medicine\/articles\/10.1177\/1535370213498975\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,4]]},"references-count":25,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1177\/1535370213498975"],"URL":"https:\/\/doi.org\/10.1177\/1535370213498975","relation":{},"ISSN":["1535-3702","1535-3699"],"issn-type":[{"value":"1535-3702","type":"print"},{"value":"1535-3699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,4]]}}}