{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:27:04Z","timestamp":1791494824104,"version":"4.3.4"},"reference-count":42,"publisher":"Frontiers Media SA","issue":"4","license":[{"start":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T00:00:00Z","timestamp":1447372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>\n                    Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB\/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB\/c male mice, around six- to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15\u2009ml of NaCl, while those in the experimental group were inoculated with 5\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-\u03b2-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the \u03b2-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.\n                  <\/jats:p>","DOI":"10.1177\/1535370215614658","type":"journal-article","created":{"date-parts":[[2015,11,14]],"date-time":"2015-11-14T20:09:06Z","timestamp":1447531746000},"page":"359-366","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?"],"prefix":"10.3389","volume":"241","author":[{"given":"A\u00edrton MC","family":"J\u00fanior","sequence":"first","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando A","family":"de Amorim Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weslany","family":"de Oliveira Dantas","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana CL","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrezza BS","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria MA","family":"de Sousa Cavalcante","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid M","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"de Deus Moura de Lima","sequence":"additional","affiliation":[{"name":"Department of Patology and Odontological Clinic, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia dos Santos","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Veterinary Surgery, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Maria","family":"de Carvalho Leite","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma Maria dos Santos","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00facia de F\u00e1tima Almeida","family":"de Deus Moura","sequence":"additional","affiliation":[{"name":"Department of Patology and Odontological Clinic, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedito B","family":"da Silva","sequence":"additional","affiliation":[{"name":"Maternal Child Departament, Federal University of Piau\u00ed, Piau\u00ed, Teresina 64049550, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2015,11,13]]},"reference":[{"key":"bibr1-1535370215614658","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-0943-2"},{"key":"bibr2-1535370215614658","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-5-1","volume":"5","author":"Silva-Almeida M","year":"2012","journal-title":"infection in mammals. 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