{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:10:18Z","timestamp":1788394218786,"version":"build-2803163510"},"reference-count":31,"publisher":"Frontiers Media SA","issue":"2","license":[{"start":{"date-parts":[[2016,7,24]],"date-time":"2016-07-24T00:00:00Z","timestamp":1469318400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Exp Biol Med (Maywood)"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>The aim of this study was to investigate the anti-hyperglycemic activity and mechanism of formononetin in alloxan-induced type 1 diabetic mice by determining its effect on some diabetes-related indices as described below. Body weight, fasting blood glucose, hepatic glycogen, serum insulin, and serum glucagon were determined by electronic scales, glucometer, and ELISA kits. Fas, Caspase-3, pancreatic and duodenal homeobox-1 , insulin receptor substrate 2, glucokinase and glucose transporter 2, mRNA and proteins levels in pancreas tissue, and glucokinase and glucose-6-phosphatase mRNA, and proteins levels in liver tissue were detected by fluorogenic quantitative-polymerase chain reaction and Western blot assays. The results indicated that formononetin (5, 10, and 20\u2009mg\/kg; oral administration) reversed the alloxan-induced increase of some indices (fasting blood glucose level and Fas and Caspase-3 mRNA and proteins levels in pancreas tissue) and reduction of some indices (body weight gain, oral glucose tolerance, insulin activity, hepatic glycogen level, pancreatic and duodenal homeobox-1, insulin receptor substrate 2, glucokinase and glucose transporter 2, mRNA and proteins levels in pancreas tissue, and glucokinase mRNA and protein levels in liver tissue). The glucagon level and glucose-6-phosphatase mRNA and protein levels in liver tissue were not affected by the drugs administration. In conclusion, formononetin exhibited anti-hyperglycemic activity in alloxan-induced type 1 diabetic mice by inhibiting islet B cell apoptosis and promoting islet B cell regeneration, insulin secretion, hepatic glycogen synthesis, and hepatic glycolysis.<\/jats:p>","DOI":"10.1177\/1535370216657445","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T02:07:38Z","timestamp":1468462058000},"page":"223-230","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":51,"title":["Formononetin exhibits anti-hyperglycemic activity in alloxan-induced type 1 diabetic mice"],"prefix":"10.3389","volume":"242","author":[{"given":"Guizhen","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Health, Linyi People\u2019s Hospital of Shandong University, Linyi 276003, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Nursing, Linyi Oncosurgical Hospital, Linyi 276002, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Huan","sequence":"additional","affiliation":[{"name":"Department of Nursing, Linyi Oncosurgical Hospital, Linyi 276002, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chinese Medicine Hospital of Linshu County, Linshu 276700, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Linyi People\u2019s Hospital of Shandong University, Linyi 276003, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2016,7,24]]},"reference":[{"key":"bibr1-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.06.016"},{"key":"bibr2-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2008.12.027"},{"key":"bibr3-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/128402"},{"key":"bibr4-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(02)00059-4"},{"key":"bibr5-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1016\/S0944-7113(11)80059-0"},{"key":"bibr6-1535370216657445","first-page":"1057","volume":"40","author":"Ma Q","year":"2005","journal-title":"Chin Pharm J"},{"key":"bibr7-1535370216657445","first-page":"3156","volume":"35","author":"Li GH","year":"2010","journal-title":"Chin J Chin Mater Med"},{"key":"bibr8-1535370216657445","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.2079"},{"key":"bibr9-1535370216657445","first-page":"17","volume":"34","author":"Yang RJ","year":"2006","journal-title":"J Northwest Sci Tech Univ Agri Nat Sci Ed"},{"key":"bibr10-1535370216657445","first-page":"535","volume":"29","author":"Chen HY","year":"2005","journal-title":"Bull Acad Mil Med Sci"},{"key":"bibr11-1535370216657445","first-page":"90","author":"Yang B","year":"2014","journal-title":"PhD thesis. 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