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The tissue bath is still used routinely as the instrument for performing the \u201cgold standard\u201d test for clinical diagnosis of malignant hyperthermia susceptibility \u2013 the caffeine-halothane contracture test. Our research group has utilized this tool for several decades for a range of research studies, and we are currently one of four North American diagnostic centers for determining susceptibility for malignant hyperthermia. This review provides a brief summary of some of the historical uses of the tissue bath. Important experimental considerations for the operation of the tissue bath are further described. 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