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Due to the conserved \u03b2-sheet-rich architecture of fibrils across various amyloid species, pan-amyloid therapeutics present a promising strategy for simultaneously targeting multiple amyloidogenic proteins. In this study, four representative amyloidogenic proteins\u2014amyloid \u03b2, serum amyloid A1, islet amyloid polypeptide, and transthyretin\u2014were selected as targets. From a library of 10,272 small molecules, five functional groups were identified as critical contributors to high-affinity binding. Utilizing these functional groups, potential compounds capable of binding to all four amyloidogenic proteins were further screened. This functional group-based screening workflow not only validates the effectiveness of functional group-driven screening in multitarget drug discovery but also facilitates the efficient identification of candidate pan-amyloid binding compounds. Furthermore, these findings provide valuable insights into the development of pan-amyloid therapeutic strategies.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1177\/1540658x261460558","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:29:13Z","timestamp":1782232153000},"page":"548-559","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Broad-Spectrum Inhibitors Targeting Multiple Amyloidogenic Proteins Using Functional Group-Based Virtual Screening"],"prefix":"10.1177","volume":"24","author":[{"given":"Jiajun","family":"Huang","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, PR China."},{"name":"Medical Innovation Research Department, PLA General Hospital, Beijing, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaohong","family":"Da","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengrui","family":"Zhai","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyan","family":"Yao","sequence":"additional","affiliation":[{"name":"Third Affiliated Hospital of Inner Mongolia Medical College, Baotou, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0694-5916","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2024.2405948"},{"issue":"10","key":"e_1_3_2_3_1","first-page":"159","article-title":"Amyloidosis\u2014the diagnosis and treatment of an underdiagnosed disease","volume":"117","author":"Ihne S","year":"2020","unstructured":"2.Ihne S, , Morbach C, , Sommer C, , Geier A, , Knop S, , St\u00f6rk S. 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