{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:36:32Z","timestamp":1792787792322,"version":"4.5.0"},"reference-count":42,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles","award":["NIH\/NCI P30CA016042"],"award-info":[{"award-number":["NIH\/NCI P30CA016042"]}],"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles","award":["P30CA016042"],"award-info":[{"award-number":["P30CA016042"]}],"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles","award":["Postdoctoral Fellowship"],"award-info":[{"award-number":["Postdoctoral Fellowship"]}],"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH","award":["GM037981"],"award-info":[{"award-number":["GM037981"]}]},{"name":"NIH","award":["GM061721"],"award-info":[{"award-number":["GM061721"]}]},{"name":"Developmental Research Program Award from the Prostate Spore","award":["NIH\/NCI P50CA092131"],"award-info":[{"award-number":["NIH\/NCI P50CA092131"]}]},{"name":"UCLA Eugene V. Cota-Robles award"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["ASSAY and Drug Development Technologies"],"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">\n                      Mitochondrial protein import is essential for overall cellular homeostasis, yet scalable approaches to systematically interrogate mitochondrial protein import and identify modulators of this process remain limited. Here, we describe a yeast-based, gain-of-growth (GoG), high-throughput screening assay for the identification of small-molecule modulators of mitochondrial protein import. In this system, truncated human proteins that contain N-terminal mitochondrial targeting sequences (MTSs) are expressed in\n                      <jats:italic toggle=\"yes\">S. cerevisiae<\/jats:italic>\n                      , where mitochondrial protein import is coupled to an auxotrophic growth readout. Disruption of import leads to cytosolic accessibility of the URA3 reporter, producing a GoG phenotype under selective conditions. As a proof of concept, we applied this model to PTEN-induced kinase 1 (PINK1), a mitochondrial imported regulator of mitochondrial quality control. Using this approach, we demonstrate the ability to monitor PINK1 import and identify candidate compounds that modulate this process. Collectively, this work establishes a scalable and reproducible platform for interrogating mitochondrial protein import and identifying compounds for downstream validation in mammalian systems.\n                    <\/jats:italic>\n                  <\/jats:p>","DOI":"10.1177\/1540658x261490394","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:39:46Z","timestamp":1790786386000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Yeast-Based High-Throughput Screening Platform for Identifying Modulators of Mitochondrial Protein Import"],"prefix":"10.1177","author":[{"given":"Maya Cielo","family":"Cornejo","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7691-8104","authenticated-orcid":false,"given":"Sandy Che-Eun S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA."},{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaya","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology and Physiology, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Taylor","sequence":"additional","affiliation":[{"name":"California NanoSystems Institute, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Damoiseux","sequence":"additional","affiliation":[{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA."},{"name":"California NanoSystems Institute, University of California, Los Angeles, California, USA."},{"name":"Department of Molecular and Medical Pharmacology, University of California Los Angeles, California, USA."},{"name":"Department of Bioengineering, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla M.","family":"Koehler","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA."},{"name":"Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA."},{"name":"Molecular Biology Institute, University of California, Los Angeles, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-120420-015303"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.06.054"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.11.001"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.66.1.863"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23063353"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300690110"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi341"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2026.184546"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA04497D"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.08.005"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914387107"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3721"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811021116"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.1405"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04363.x"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.17"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.770131"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.130765"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.70009"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2005.03.004"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201008084"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000298"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096284"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi377"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/122.1.19"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90454-W"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)94029-C"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17219"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.17.5408"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1998.4.143"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05446.x"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607773104"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq526"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3028"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.17191"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-05-0272"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.630551"},{"key":"e_1_3_3_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.123407"},{"key":"e_1_3_3_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30305-z"},{"key":"e_1_3_3_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)54076-9"},{"key":"e_1_3_3_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"key":"e_1_3_3_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.15.4819"}],"container-title":["ASSAY and Drug Development Technologies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1540658X261490394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1540658X261490394","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1540658X261490394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:17:18Z","timestamp":1791044238000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1540658X261490394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":42,"alternative-id":["10.1177\/1540658X261490394"],"URL":"https:\/\/doi.org\/10.1177\/1540658x261490394","relation":{},"ISSN":["1540-658X","1557-8127"],"issn-type":[{"value":"1540-658X","type":"print"},{"value":"1557-8127","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"article-number":"1540658X261490394"}}