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To pursue this goal, we dissociated porcine third molar tooth buds into single-cell suspensions and seeded them onto biodegradable polymers. After growing in rat hosts for 20 to 30 weeks, recognizable tooth structures formed that contained dentin, odontoblasts, a well-defined pulp chamber, putative Hertwig\u2019s root sheath epithelia, putative cementoblasts, and a morphologically correct enamel organ containing fully formed enamel. Our results demonstrate the first successful generation of tooth crowns from dissociated tooth tissues that contain both dentin and enamel, and suggest the presence of epithelial and mesenchymal dental stem cells in porcine third molar tissues.<\/jats:p>","DOI":"10.1177\/154405910208101008","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"695-700","source":"Crossref","is-referenced-by-count":387,"title":["Tissue Engineering of Complex Tooth Structures on Biodegradable Polymer Scaffolds"],"prefix":"10.1177","volume":"81","author":[{"given":"C.S.","family":"Young","sequence":"first","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.P.","family":"Vacanti","sequence":"additional","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.D.","family":"Bartlett","sequence":"additional","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.C.","family":"Yelick","sequence":"additional","affiliation":[{"name":"Department of Cytokine Biology and Harvard-Forsyth Department of Oral Biology, The Forsyth Institute, Boston, MA 02115, USA;"},{"name":"Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; and"},{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,10,1]]},"reference":[{"key":"BARTLETT-AND-SIMMER-1999","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100040101"},{"key":"BOHL-ETAL-1998","doi-asserted-by":"publisher","DOI":"10.1163\/156856298X00127"},{"key":"BRONCKERS-ETAL-1993","doi-asserted-by":"publisher","DOI":"10.1007\/BF00302729"},{"key":"CHEN-ETAL-1993","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(93)90034-J"},{"key":"CHOI-AND-VACANTI-1997","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(96)00164-9"},{"key":"DEAKINS-AND-VOLKER-1941","doi-asserted-by":"publisher","DOI":"10.1177\/00220345410200020201"},{"key":"DIEKWISCH-2001","unstructured":"Diekwisch TG -2001- The developmental biology of cementum. 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