{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:32:28Z","timestamp":1777811548944,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>\n                    Through a mechanism that is unclear, systemic fluoroquinolones and tetracyclines can attain higher levels in gingival fluid than in blood. We hypothesized that gingival fibroblasts take up and accumulate these agents, thereby enhancing their redistribution to the gingiva. Using fluorescence to monitor transport activity, we characterized the accumulation of fluoroquinolones and tetracyclines in cultured human gingival fibroblast monolayers. Both were transported in a concentrative, temperature-dependent, and saturable manner. Fibroblasts transported ciprofloxacin and minocycline with K\n                    <jats:sub>m<\/jats:sub>\n                    values of 200 and 108 \u03bcg\/mL, respectively, at maximum velocities of 4.62 and 14.2 ng\/min\/\u03bcg cell protein, respectively. For both agents, transport was most efficient at pH 7.2 and less efficient at pH 6.2 and 8.2. At steady state, the cellular\/extracellular concentration ratio was &gt; 8 for ciprofloxacin and &gt; 60 for minocycline. Thus, gingival fibroblasts possess active transporters that could potentially contribute to the relatively high levels these agents attain in gingival fluid.\n                  <\/jats:p>","DOI":"10.1177\/154405910208101208","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"836-840","source":"Crossref","is-referenced-by-count":15,"title":["Accumulation of Ciprofloxacin and Minocycline by Cultured Human Gingival                Fibroblasts"],"prefix":"10.1177","volume":"81","author":[{"given":"Q.","family":"Yang","sequence":"first","affiliation":[{"name":"Sections of Oral Biology and"},{"name":"Periodontology, College of Dentistry, The Ohio State University\r               Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH\r               43218-2357;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.J.","family":"Nakkula","sequence":"additional","affiliation":[{"name":"Sections of Oral Biology and"},{"name":"Periodontology, College of Dentistry, The Ohio State University\r               Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH\r               43218-2357;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.D.","family":"Walters","sequence":"additional","affiliation":[{"name":"Sections of Oral Biology and"},{"name":"Periodontology, College of Dentistry, The Ohio State University\r               Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH\r               43218-2357;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,12,1]]},"reference":[{"key":"BOUNDS-ETAL-2000","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.10.2609-2614.2000"},{"key":"BRADFORD-1976","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"CHRISTERSSON-ETAL-1987","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1987.58.8.529"},{"key":"CIANCIO-ETAL-1980","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1980.51.9.530"},{"key":"CONWAY-ETAL-2000","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.9.1448"},{"key":"DRESSER-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Dresser MJ, Zhang L, Giacomini KM\n               -1999- Molecular and functional characteristics of cloned human organic\n               cation transporters. 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