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Water sorption is thought to be nonuniform and can be traced by the use of silver nitrate. Human teeth bonded with an experimental filled-adhesive were aged in artificial saliva (experimental) or non-aqueous mineral oil (control). Specimens retrieved for up to a 12-month period were immersed in 50 wt% ammoniacal silver nitrate and examined by transmission electron microscopy for identification of the changes in their silver uptake. Reticular silver deposits initially identified within the bulk of hybrid layers in the experimental group were gradually reduced over time, but were subsequently replaced by similar deposits that were located along the hybrid layer-adhesive interface. Silver uptake in water-binding domains of the adhesive layers increased with aging, resulting in water tree formation. These water-filled channels may act as potential sites for hydrolytic degradation of resin-dentin bonds.<\/jats:p>","DOI":"10.1177\/154405910308200710","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"537-541","source":"Crossref","is-referenced-by-count":152,"title":["Aging Affects Two Modes of Nanoleakage Expression in Bonded Dentin"],"prefix":"10.1177","volume":"82","author":[{"given":"F.R.","family":"Tay","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, University of Hong Kong, Hong Kong SAR, China;"},{"name":"Division of Pediatric Dentistry, Hokkaido University, Graduate School of Dental Medicine, Sapporo, Hokkaido, Japan;"},{"name":"Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1129, USA; and"},{"name":"Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, University of Hong Kong, Hong Kong SAR, China;"},{"name":"Division of Pediatric Dentistry, Hokkaido University, Graduate School of Dental Medicine, Sapporo, Hokkaido, Japan;"},{"name":"Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1129, USA; 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