{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:20:07Z","timestamp":1777810807197,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:p>Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important virulence factors of the cariogenic organism Streptococcus mutans. They may mediate sucrose-independent or sucrose-dependent attachment of Streptococcus mutans to tooth surfaces, respectively. Thus, inhibiting both virulence factors is predicted to provide better protection against caries than inhibiting a single factor. To develop a highly efficient vaccine against caries, we constructed a fusion DNA vaccine, pGLUA-P, by cloning the GLU region of GTF into a DNA vaccine, pCIA-P, which encodes two highly conservative regions of PAc. In this report, we provide evidence that fewer caries lesions were observed in rats following subcutaneous injection of pGLUA-P, compared with pCIA-P, near the submandibular gland. Our findings suggest that a multigenic DNA vaccine may be more caries-preventive than a single-gene DNA vaccine.<\/jats:p>","DOI":"10.1177\/154405910408300316","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"266-270","source":"Crossref","is-referenced-by-count":29,"title":["Construction and Immunogenic Characterization of a Fusion Anti-caries DNA                Vaccine against PAc and Glucosyltransferase I of\n                    <i>Streptococcus                   mutans<\/i>"],"prefix":"10.1177","volume":"83","author":[{"given":"J.H.","family":"Guo","sequence":"first","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.W.","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Bian","sequence":"additional","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,3,1]]},"reference":[{"key":"CHIA-ETAL-1993","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.11.4689-4695.1993"},{"key":"COPE-AND-MOOSER-1993","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.11.4814-4817.1993"},{"key":"FAN-ETAL-2002","doi-asserted-by":"publisher","DOI":"10.1177\/0810784"},{"key":"GALVIN-ETAL-2000","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00569-1"},{"key":"HAMADA-AND-SLADE-1980","doi-asserted-by":"publisher","DOI":"10.1128\/mr.44.2.331-384.1980"},{"key":"HAMADA-ETAL-1989","doi-asserted-by":"crossref","unstructured":"Hamada S, Horikoshi T, Minami T, Okahashi N, Koga T\n                  -1989- Purification characterization of cell-associated\n               glucosyltransferase synthesizing water-insoluble glucan from serotype c\n                      Streptococcus mutans\n                      . 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